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南方医科大学附属广东省人民医院内分泌科,广州 510080
龙 倩,Email:qianl606@163.com,研究方向:内分泌与代谢病。
余超伦,Email:yuchaolun@gdph.org.cn。
收稿:2025-08-14,
修回:2025-10-29,
录用:2025-11-03,
纸质出版:2025-12-28
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龙倩,关海霞,余超伦.甲状腺激素受体激动剂对大鼠慢性肾纤维化的作用研究[J].重庆医科大学学报,2025,50(12):1620-1625.
Long Qian,Guan Haixia,Yu Chaolun.Effects of thyroid hormone receptor-β agonist on chronic renal fibrosis in 5/6 nephrectomized rats[J].Journal of Chongqing Medical University,2025,50(12):1620-1625.
龙倩,关海霞,余超伦.甲状腺激素受体激动剂对大鼠慢性肾纤维化的作用研究[J].重庆医科大学学报,2025,50(12):1620-1625. DOI: 10.13406/j.cnki.cyxb.003980.
Long Qian,Guan Haixia,Yu Chaolun.Effects of thyroid hormone receptor-β agonist on chronic renal fibrosis in 5/6 nephrectomized rats[J].Journal of Chongqing Medical University,2025,50(12):1620-1625. DOI: 10.13406/j.cnki.cyxb.003980.
目的
2
研究甲状腺激素受体β(thyroid hormone receptor-β,THR-β)激动剂对5/6肾切除大鼠模型慢性肾纤维化的作用。
方法
2
将构建的5/6肾切除大鼠模型分为对照组与THR-β激动剂实验组,每组6只。对照组每日腹腔注射1 mL生理盐水;实验组每日腹腔注射含THR-β激动剂1 μg/(g·d)的溶剂1 mL,持续给药10周。
结果
2
与对照组比较,THR-β激动剂组大鼠在5/6肾切除第2~6周血肌酐水平有改善(
P
<
0.05),第10周时体质量增加(
P
<
0.05),而血脂水平降低(甘油三酯
P<
0.01,低密度脂蛋白
P<
0.001),胆红素水平也下降(总胆红素与直接胆红素,均
P
<
0.05)。2组大鼠肾脏组织的Masson染色与α-平滑肌肌动蛋白(alpha-smooth muscle actin,α-SMA)免疫组化染色结果显示,纤维化未见明显差异;THR-β激动剂组的增殖细胞核抗原(proliferating cell nuclear antigen,PCNA)免疫组化染色阳性细胞数增多,提示肾小管细胞增殖活跃。
结论
2
THR-β激动剂未能明显改善5/6肾切除大鼠的慢性肾脏纤维化,但可能具有促进肾小管细胞增殖的作用。
Objective
2
To investigate the effect of thyroid hormone receptor-β(THR-β) agonist on chronic renal fibrosis in 5/6 nephrectomized rats.
Methods
2
A rat model of 5/6 nephrectomy was established,and the rats were divided into control group and THR-β agonist-treated experimental group,with 6 rats in each group. The rats in the control group were given intraperitoneal injection of 1 mL saline every day,while those in the experimental group were given intraperitoneal injection of 1 mL THR-β agonist every day 1 μg/(g·d);both groups were treated for 10 consecutive weeks.
Results
2
Compared with the control group,the experimental group had a significant improvement in serum creatinine at weeks 2-6 after 5/6 nephrectomy(
P
<
0.05),as well as a significant increase in body weight(
P
<
0.05) and significant reductions in blood lipids(
P
<
0.01 for triglycerides and
P
<
0.001 for low-density lipoprotein) and bilirubin(
P
<
0.05 for both total bilirubin and direct bilirubin) at week 10. Masson staining of renal tissue and immunohistochemical staining for alpha-smooth muscle actin showed no significant difference in renal fibrosis between the two groups,while for the experimental group,immunohistochemical staining for proliferating cell nuclear antigen(PCNA) showed an increase in the number of PCNA-positive cells and active proliferation of renal tubular cells.
Conclusion
2
THR-β agonist cannot significantly improve chronic renal fibrosis in 5/6 nephrectomized rats,but it can promote the proliferation of renal tubular cells.
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