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口腔疾病防治全国重点实验室/国家口腔疾病临床医学研究中心/四川大学华西口腔医院牙体牙髓病科,成都 610041
陈 雨,Email:chenyu_zzZ@outlook.com,研究方向:牙体硬组织修复及功能材料研究。
张凌琳,Email;zhanglinglin@scu.edu.cn。
收稿:2025-02-25,
修回:2025-03-11,
录用:2025-03-14,
纸质出版:2025-09-28
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陈雨,王雨霏,张凌琳.介孔生物活性玻璃对牙菌斑生物膜组成及功能的调控作用研究[J].重庆医科大学学报,2025,50(9):1261-1270.
Chen Yu,Wang Yufei,Zhang Linglin.Effects of mesoporous bioactive glass on the composition and function of dental plaque biofilm[J].Journal of Chongqing Medical University,2025,50(9):1261-1270.
陈雨,王雨霏,张凌琳.介孔生物活性玻璃对牙菌斑生物膜组成及功能的调控作用研究[J].重庆医科大学学报,2025,50(9):1261-1270. DOI: 10.13406/j.cnki.cyxb.003848.
Chen Yu,Wang Yufei,Zhang Linglin.Effects of mesoporous bioactive glass on the composition and function of dental plaque biofilm[J].Journal of Chongqing Medical University,2025,50(9):1261-1270. DOI: 10.13406/j.cnki.cyxb.003848.
目的
2
牙菌斑生物膜的组成与功能易受影响,可能引发各种口腔疾病。介孔生物活性玻璃(mesoporous bioactive glass,MBG)用途广泛,但其如何影响口腔牙菌斑生物膜组成及功能尚不清楚。本研究的目的在于探究MBG对体外牙菌斑生物膜中的活菌组成及生物功能的影响。
方法
2
合成表征MBG,继而使用MBG短期处理体外牙菌斑生物膜,进行CFU计数评价其对生物膜生物量的影响。收集MBG处理后的牙菌斑样本进行宏转录组学研究,分析物种组成并筛选差异基因。最后通过RT-qPCR验证核心差异基因表达。
结果
2
CFU计数结果显示MBG短期处理减少了44.25%的体外牙菌斑生物膜生物量;宏转录组结果显示MBG明显改变了牙菌斑生物膜的物种构成,共生链球菌及克雷伯菌为主要优势物种,并且主要上调其碳水化合物代谢功能,上调基因主要富集在三羧酸循环通路。RT-qPCR显示共有9个三羧酸循环基因表达增强。
结论
2
本研究证实了MBG能显著改变体外牙菌斑生物膜的物种组成结构,增强体外牙菌斑生物膜的碳水化合物代谢,为MBG在口腔领域的应用提供了新的理论基础。
Objective
2
The composition and function of dental plaque biofilm are susceptible to alterations,potentially leading to various oral diseases. Although mesoporous bioactive glass(MBG) has been widely used,how it affects the microbial composition and biological function of dental plaque biofilm remains unclear. This study aims to investigate the effects of MBG on the composition and biological function of viable bacteria in dental plaque in vitro.
Methods
2
At first,MBG was synthesized and characterized. Subsequently,the MBG was applied to dental plaque biofilm in vitro for a short term. The impact on biofilm biomass was assessed through colony-forming unit(CFU) counting. MBG-treated samples were collected for metatranscriptomic analyses of species composition and differentially expressed genes. Finally,reverse transcription quantitative polymerase chain reaction(RT-qPCR) was performed to validate the expression of core DEGs.
Results
2
The results of CFU counting showed that short-term MBG treatment reduced dental plaque biofilm biomass in vitro by 44.25 %. The results of metatranscriptomics showed that MBG significantly changed the species composition of dental plaque biofilm,where commensal
Streptococci
and
Klebsiella
were predominant. The function of carbohydrate metabolism was up-regulated,and the up-regulated genes were mainly enriched in the tricarboxylic acid(TCA) cycle. The results of RT-qPCR showed that a total of 9 genes associated with the TCA cycle were up-regulated.
Conclusion
2
MBG can affect the species composition and enhance the carbohydrate metabolism of dental plaque biofilm in vitro,providing a new theoretical basis for the application of MBG in the oral field.
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