
浏览全部资源
扫码关注微信
重庆医科大学公共卫生学院生殖与发育教育部国际合作联合实验室,重庆 400016
王 静,Email:wang-jing@stu.cqmu.edu.cn,研究方向:环境暴露对生殖功能的影响。
李伟轲,Email:liweike@cqmu.edu.cn。
收稿:2024-09-03,
修回:2024-10-23,
录用:2024-11-05,
纸质出版:2025-07-28
移动端阅览
王静,刁家妮,龙菁,等.聚苯乙烯微塑料联合高脂处理对血管内皮细胞的影响[J].重庆医科大学学报,2025,50(7):969-976.
Wang Jing,Diao Jiani,Long Jing,et al.Effect of polystyrene microplastics combined with high-fat treatment on vascular endothelial cells[J].Journal of Chongqing Medical University,2025,50(7):969-976.
王静,刁家妮,龙菁,等.聚苯乙烯微塑料联合高脂处理对血管内皮细胞的影响[J].重庆医科大学学报,2025,50(7):969-976. DOI: 10.13406/j.cnki.cyxb.003734.
Wang Jing,Diao Jiani,Long Jing,et al.Effect of polystyrene microplastics combined with high-fat treatment on vascular endothelial cells[J].Journal of Chongqing Medical University,2025,50(7):969-976. DOI: 10.13406/j.cnki.cyxb.003734.
目的
2
探讨聚苯乙烯微塑料(polystyrene microplastics,PS-MPs)联合高脂处理对血管内皮细胞的影响。
方法
2
使用含5%胎牛血清的DMEM培养基培养人脐静脉血管内皮细胞(human umbilical vein endothelial cells,HUVECs)。对HUVECs采用常规培养、高脂处理和PS-MPs联合高脂处理。实验分为对照组、高脂处理组和PS-MPs联合高脂处理组。采用CCK-8试剂检测细胞活力,F-actin染色检测细胞形态变化情况,流式细胞术、划痕实验和管腔形成实验检测细胞凋亡、迁移和成管能力。
结果
2
HUVECs暴露于高脂环境后,细胞活力明显降低,细胞形态皱缩,细胞凋亡水平明显增加,细胞迁移和成管能力均明显降低。相较于高脂处理组,PS-MPs联合高脂处理后,细胞活力,细胞形态,细胞凋亡水平和细胞迁移能力均没有明显变化,但细胞成管能力进一步受损。
结论
2
高脂处理会影响细胞活力,改变细胞形态,损伤血管内皮细胞功能;在高脂处理基础上联合PS-MPs处理后,可加剧血管内皮细胞功能损伤。
Objective
2
To investigate the effect of polystyrene microplastics(PS-MPs) combined with high-fat treatment on vascular endothelial cells.
Methods
2
Human umbilical vein endothelial cells(HUVECs) were cultured in the DMEM medium containing 5% fetal bovine serum. HUVECs were treated with conventional culture,high-fat treatment,and PS-MPs combined with high-fat treatment. The experiment was conducted in the three groups of control group,high-fat treatment group and PS-MPs + high-fat treatment group. CCK-8 assay was used to measure cell viability,F-actin staining was used to observe cell morphological changes,and flow cytometry,scratch assay,and tube formation assay were used to measure the apoptosis,migration,and tube-forming ability of cells.
Results
2
After HUVECs were exposed to the high-fat environment,there was a significant reduction in cell viability,shrinkage of cells,a significant increase in cell apoptosis,and significant reductions in cell migration and tube-forming ability. Compared with the high-fat treatment group,there were no significant changes in cell viability,cell morphology,cell apoptosis,and cell migration ability after PS-MPs combined with high-fat treatment,but the tube-forming ability of cells was further impaired.
Conclusion
2
High-fat treatment will affect cell viability,change cell morphology,and damage vascular endothelial cell function,and PS-MPs combined with high-fat treatment can aggravate the damage of vascular endothelial cell function.
Antoniotti GS , Coughlan M , Salamonsen LA , et al . Obesity associated advanced glycation end products within the human uterine cavity adversely impact endometrial function and embryo implantation competence [J ] . Hum Reprod , 2018 , 33 ( 4 ): 654 - 665 .
Rhee JS , Saben JL , Mayer AL , et al . Diet-induced obesity impairs endometrial stromal cell decidualization:a potential role for impaired autophagy [J ] . Hum Reprod , 2016 , 31 ( 6 ): 1315 - 1326 .
Tripathy D , Mohanty P , Dhindsa S , et al . Elevation of free fatty acids induces inflammation and impairs vascular reactivity in healthy subjects [J ] . Diabetes , 2003 , 52 ( 12 ): 2882 - 2887 .
Steinberg HO , Tarshoby M , Monestel R , et al . Elevated circulating free fatty acid levels impair endothelium-dependent vasodilation [J ] . J Clin Invest , 1997 , 100 ( 5 ): 1230 - 1239 .
Inoguchi T , Li P , Umeda F , et al . High glucose level and free fatty acid stimulate reactive oxygen species production through protein kinase C:dependent activation of NAD(P)H oxidase in cultured vascular cells [J ] . Diabetes , 2000 , 49 ( 11 ): 1939 - 1945 .
龙菁 . 子宫内膜基质细胞前列腺素E2在肥胖导致的蜕膜血管生成障碍中的作用及机制研究 [D ] . 重庆 : 重庆医科大学 , 2022 .
Long J . The role and mechanism of prostaglandin E2 in endometrial stromal cells in obesity induced decidual angiogenesis disorders [D ] . Chongqing : Chongqing Medical University , 2022 .
郝晓娟 . 血管内皮细胞功能的影响因素及其研究进展 [J ] . 医学综述 , 2012 , 18 ( 20 ): 3371 - 3374 .
Hao XJ . Research progress of influencing factors on the function of vascular endothelial cells [J ] . Med Recapitul , 2012 , 18 ( 20 ): 3371 - 3374 .
Potente M , Gerhardt H , Carmeliet P . Basic and therapeutic aspects of angiogenesis [J ] . Cell , 2011 , 146 ( 6 ): 873 - 887 .
宗如月 , 罗新鹏 , 路小超 , 等 . 血管生成在恶性肿瘤中作用及靶向治疗策略的研究进展 [J ] . 生命科学 , 2022 , 8 ( 6 ): 644 - 652 .
Zong RY , Luo XP , Lu XC , et al . Progress of angiogenesis and targeted therapy strategies in human cancer [J ] . Chin Bull Life Sci , 2022 , 8 ( 6 ): 644 - 652 .
Triggle CR , Samuel SM , Ravishankar S , et al . The endothelium:influencing vascular smooth muscle in many ways [J ] . Can J Physiol Pharmacol , 2012 , 90 ( 6 ): 713 - 738 .
Santos-Gomes J , Le Ribeuz H , Brás-Silva C , et al . Role of ion channel remodeling in endothelial dysfunction induced by pulmonary arterial hypertension [J ] . Biomolecules , 2022 , 12 ( 4 ): 484 .
Mehrotra D , Wu JX , Papangeli I , et al . Endothelium as a gatekeeper of fatty acid transport [J ] . Trends Endocrinol Metab , 2014 , 25 ( 2 ): 99 - 106 .
Barnes DKA , Galgani F , Thompson RC , et al . Accumulation and fragmentation of plastic debris in global environments [J ] . Philos Trans R Soc Lond B Biol Sci , 2009 , 364 ( 1526 ): 1985 - 1998 .
Yousif E , Haddad R . Photodegradation and photostabilization of polymers,especially polystyrene:review [J ] . Springerplus , 2013 , 2 : 398 .
Auta HS , Emenike CU , Fauziah SH . Distribution and importance of microplastics in the marine environment:a review of the sources,fate,effects,and potential solutions [J ] . Environ Int , 2017 , 102 : 165 - 176 .
Kannan K , Vimalkumar K . A review of human exposure to microplastics and insights into microplastics as obesogens [J ] . Front Endocrinol , 2021 , 12 : 724989 .
Wang J , Lv SH , Zhang MY , et al . Effects of plastic film residues on occurrence of phthalates and microbial activity in soils [J ] . Chemosphere , 2016 , 151 : 171 - 177 .
Dong YM , Gao ML , Qiu WW , et al . Uptake of microplastics by carrots in presence of As (III):Combined toxic effects [J ] . J Hazard Mater , 2021 , 411 : 125055 .
An D , Na J , Song J , et al . Size-dependent chronic toxicity of fragmented polyethylene microplastics to Daphnia magna [J ] . Chemosphere , 2021 , 271 : 129591 .
Ibrahim YS , Tuan Anuar S , Azmi AA , et al . Detection of microplastics in human colectomy specimens [J ] . JGH Open , 2020 , 5 ( 1 ): 116 - 121 .
Ragusa A , Svelato A , Santacroce C , et al . Plasticenta:First evidence of microplastics in human placenta [J ] . Environ Int , 2021 , 146 : 106274 .
Schwabl P , Köppel S , Königshofer P , et al . Detection of various microplastics in human stool:a prospective case series [J ] . Ann Intern Med , 2019 , 171 ( 7 ): 453 - 457 .
Chen YC , Chen KF , Lin KY A , et al . Evaluation of toxicity of polystyrene microplastics under realistic exposure levels in human vascular endothelial EA. hy926 cells [J ] . Chemosphere , 2023 , 313 : 137582 .
Geyer R , Jambeck JR , Law KL . Production,use,and fate of all plastics ever made [J ] . Sci Adv , 2017 , 3 ( 7 ): e1700782 .
Zhu XQ , Wang CX , Duan XY , et al . Micro- and nanoplastics:a new cardiovascular risk factor? [J ] . Environ Int , 2023 , 171 : 107662 .
Li ZK , Zhu SX , Liu Q , et al . Polystyrene microplastics cause cardiac fibrosis by activating Wnt/β-catenin signaling pathway and promoting cardiomyocyte apoptosis in rats [J ] . Environ Pollut , 2020 , 265 ( Pt A ): 115025 .
火传益 , 张 慧 , 倪玲 , 等 . 亚低温对聚苯乙烯微塑料所致血管内皮细胞损伤的影响 [J ] . 环境卫生学杂志 , 2023 , 13 ( 4 ): 250 - 256 .
Huo CY , Zhang H , Ni L , et al . Effects of mild hypothermia on vascular endothelial cell injury induced by polystyrene microplastics [J ] . J Environ Hyg , 2023 , 13 ( 4 ): 250 - 256 .
Lu YY , Li HY , Ren HY , et al . Size-dependent effects of polystyrene nanoplastics on autophagy response in human umbilical vein endothelial cells [J ] . J Hazard Mater , 2022 , 421 : 126770 .
Bhagat J , Zang LQ , Nakayama H , et al . Effects of nanoplastic on toxicity of azole fungicides (ketoconazole and fluconazole) in zebrafish embryos [J ] . Sci Total Environ , 2021 , 800 : 149463 .
Wang QP , Li YZ , Chen YR , et al . Toxic effects of polystyrene nanoplastics and polybrominated diphenyl ethers to zebrafish ( Danio rerio ) [J ] . Fish Shellfish Immunol , 2022 , 126 : 21 - 33 .
Du J , Hu YQ , Hou MM , et al . Combined effects of high-fat diet and polystyrene microplastic exposure on microplastic bioaccumulation and lipid metabolism in zebrafish [J ] . Fish Shellfish Immunol , 2023 , 137 : 108803 .
Hetherington AM , Sawyez CG , Zilberman E , et al . Differential lipotoxic effects of palmitate and oleate in activated human hepatic stellate cells and epithelial hepatoma cells [J ] . Cell Physiol Biochem , 2016 , 39 ( 4 ): 1648 - 1662 .
Lee HS , Amarakoon D , Wei CI , et al . Adverse effect of polystyrene microplastics(PS-MPs) on tube formation and viability of human umbilical vein endothelial cells [J ] . Food Chem Toxicol , 2021 , 154 : 112356 .
Davis S , Aldrich TH , Jones PF , et al . Isolation of angiopoietin-1,a ligand for the TIE2 receptor,by secretion-trap expression cloning [J ] . Cell , 1996 , 87 ( 7 ): 1161 - 1169 .
Sun Q , Ren SY , Ni HG . Incidence of microplastics in personal care products:an appreciable part of plastic pollution [J ] . Sci Total Environ , 2020 , 742 : 140218 .
Amato-Lourenço LF , Carvalho-Oliveira R , Júnior GR , et al . Presence of airborne microplastics in human lung tissue [J ] . J Hazard Mater , 2021 , 416 : 126124 .
Li SL , Qiao ZP , Huang MD , et al . Combined exposure of polystyrene microplastics and benzo[a]pyrene in rat:Study of the oxidative stress effects in the liver [J ] . Ecotoxicol Environ Saf , 2024 , 278 : 116390 .
Rainieri S , Conlledo N , Larsen BK , et al . Combined effects of microplastics and chemical contaminants on the organ toxicity of zebrafish ( Danio rerio ) [J ] . Environ Res , 2018 , 162 : 135 - 143 .
缪立颖 , 王 欣 , 韩洋洋 . 泛凋亡在肝脏疾病中的作用及其机制研究进展 [J ] . 中华肝脏病杂志 , 2024 , 32 ( 11 ): 1047 - 1052 .
Miao LY , Wang X , Han YY . Research progress on the role and mechanism of PANoptosis in liver diseases [J ] . Chin J Hepatol , 2024 , 32 ( 11 ): 1047 - 1052 .
Korner J , Aronne LJ . The emerging science of body weight regulation and its impact on obesity treatment [J ] . J Clin Invest , 2003 , 111 ( 5 ): 565 - 570 .
Madamanchi NR , Vendrov A , Runge MS . Oxidative stress and vascular disease [J ] . Arterioscler Thromb Vasc Biol , 2005 , 25 ( 1 ): 29 - 38 .
Rocha VZ , Libby P . Obesity,inflammation,and atherosclerosis [J ] . Nat Rev Cardiol , 2009 , 6 : 399 - 409 .
Hirosumi J , Tuncman G , Chang LF , et al . A central role for JNK in obesity and insulin resistance [J ] . Nature , 2002 , 420 ( 6913 ): 333 - 336 .
Goeckeler ZM , Wysolmerski RB . Myosin light chain kinase-regulated endothelial cell contraction:the relationship between isometric tension,actin polymerization,and myosin phosphorylation [J ] . J Cell Biol , 1995 , 130 ( 3 ): 613 - 627 .
He F , Peng J , Deng XL , et al . RhoA and NF-κB are involved in lipopolysaccharide-induced brain microvascular cell line hyperpermeability [J ] . Neuroscience , 2011 , 188 : 35 - 47 .
Kim JY , Lee YG , Kim MY , et al . Src-mediated regulation of inflammatory responses by actin polymerization [J ] . Biochem Pharmacol , 2010 , 79 ( 3 ): 431 - 443 .
Koss M , 2nd Pfeiffer GR , Wang Y , et al . Ezrin/radixin/moesin proteins are phosphorylated by TNF-alpha and modulate permeability increases in human pulmonary microvascular endothelial cells [J ] . J Immunol , 2006 , 176 ( 2 ): 1218 - 1227 .
Rizov M , Andreeva P , Dimova I . Molecular regulation and role of angiogenesis in reproduction [J ] . J Obstet Gynecol , 2017 , 56 ( 2 ): 127 - 132 .
Tsakiroglou P , VandenAkker NE , Del Bo' C , et al . Role of berry anthocyanins and phenolic acids on cell migration and angiogenesis:an updated overview [J ] . Nutrients , 2019 , 11 ( 5 ): 1075 .
李迎敏 , 阴怀清 , 栗 红 , 等 . 亚低温干预下新生大鼠缺氧缺血性脑损伤脑组织Bax基因表达变化及意义 [J ] . 中西医结合心脑血管病杂志 , 2014 , 12 ( 9 ): 1114 - 1116 .
Li YM , Yin HQ , Li H , et al . Bax gene expression after hypoxic ischemic brain injury by mild hypothemia intervention in neonatal rats [J ] . Chin J Integr Med Cardio Cerebrovasc Dis , 2014 , 12 ( 9 ): 1114 - 1116 .
Nakagami H , Morishita R , Yamamoto K , et al . Phosphorylation of p38 mitogen-activated protein kinase downstream of bax-caspase-3 pathway leads to cell death induced by high D-glucose in human endothelial cells [J ] . Diabetes , 2001 , 50 ( 6 ): 1472 - 1481 .
Nowak-Sliwinska P , Alitalo K , Allen E , et al . Consensus guidelines for the use and interpretation of angiogenesis assays [J ] . Angiogenesis , 2018 , 21 ( 3 ): 425 - 532 .
Suen JL , Chang Y , Shiu YS , et al . IL-10 from plasmacytoid dendritic cells promotes angiogenesis in the early stage of endometriosis [J ] . J Pathol , 2019 , 249 ( 4 ): 485 - 497 .
Liao FC , Liao ZQ , Zhang T , et al . ECFC-derived exosomal THBS1 mediates angiogenesis and osteogenesis in distraction osteogenesis via the PI3K/AKT/ERK pathway [J ] . J Orthop Translat , 2022 , 37 : 12 - 22 .
Fallah A , Sadeghinia A , Kahroba H , et al . Therapeutic targeting of angiogenesis molecular pathways in angiogenesis-dependent diseases [J ] . Biomed Pharmacother , 2019 , 110 : 775 - 785 .
Tan HW , Yue TT , Xu Y , et al . Microplastics reduce lipid digestion in simulated human gastrointestinal system [J ] . Environ Sci Technol , 2020 , 54 ( 19 ): 12285 - 12294 .
0
浏览量
4
下载量
0
CSCD
关联资源
相关文章
相关作者
相关机构
京公网安备11010802024621