浏览全部资源
扫码关注微信
非小细胞肺癌血清lncRNA XIST、miR-126-3p表达与根治术后复发的关系
Relationship between the expression of serum lncRNA XIST and miR-126-3p in non-small cell lung cancer and recurrence after radical surgery
- 局解手术学杂志 2025年34卷第2期 页码：134-138
- 作者机构：
1.中国人民解放军南部战区总医院心胸外科，广东 广州 510030
2.中国人民解放军陆军第七十四集团军医院神经内科，广东 广州 510220
- 作者简介：
苏凯，E-mail：j38xkn@163.com
- 基金信息：2020年广东省医学科学技术研究基金项目(A2020452)
DOI：10.11659/jjssx.03E024070
中图分类号： R734.2纸质出版日期：2025-02-25，
收稿日期：2024-03-12，
- 稿件说明：
移动端阅览
苏凯, 俞庆华, 曹家旺, 等. 非小细胞肺癌血清lncRNA XIST、miR-126-3p表达与根治术后复发的关系[J]. 局解手术学杂志, 2025,34(2):134-138.
SU KAI, YU QING-HUA, CAO JIA-WANG, et al. Relationship between the expression of serum lncRNA XIST and miR-126-3p in non-small cell lung cancer and recurrence after radical surgery. [J]. Journal of regional anatomy and operative surgery, 2025, 34(2): 134-138.
苏凯, 俞庆华, 曹家旺, 等. 非小细胞肺癌血清lncRNA XIST、miR-126-3p表达与根治术后复发的关系[J]. 局解手术学杂志, 2025,34(2):134-138. DOI： 10.11659/jjssx.03E024070.
SU KAI, YU QING-HUA, CAO JIA-WANG, et al. Relationship between the expression of serum lncRNA XIST and miR-126-3p in non-small cell lung cancer and recurrence after radical surgery. [J]. Journal of regional anatomy and operative surgery, 2025, 34(2): 134-138. DOI： 10.11659/jjssx.03E024070.
摘要
目的
2
探讨非小细胞肺癌（NSCLC）患者血清长链非编码RNA X无活性特异性转录物（lncRNA XIST）、微小RNA-126-3p（miR-126-3p）表达及其与根治术后复发的关系。
方法
2
选取2019年2月至2020年10月中国人民解放军南部战区总医院收治的108例行根治术的NSCLC患者作为NSCLC组，选取同期在该院体检的健康志愿者52例作为对照组。采用qRT-PCR法检测血清lncRNA XIST、miR-126-3p相对表达水平；
Pearson
法分析血清lncRNA XIST与miR-126-3p表达的相关性；
Logistic
回归分析NSCLC患者术后复发的影响因素；受试者工作特征（
ROC
）曲线评估血清lncRNA XIST、miR-126-3p对患者术后复发的预测价值。
结果
2
NSCLC组患者血清lncRNA XIST表达水平高于对照组（
P
<
0.05），miR-126-3p表达水平则低于对照组（
P
<
0.05）。血清lncRNA XIST、miR-126-3p表达水平与TNM分期、淋巴结转移有关（
P
<
0.05）。生物信息学网站预测显示，lncRNA XIST与miR-126-3p存在靶向结合位点，且血清lncRNA XIST与miR-126-3p表达呈负相关（
r
=-0.579，
P
<
0.05）。与未复发组相比，复发组患者血清lncRNA XIST表达水平升高（
P
<
0.05），miR-126-3p表达水平降低（
P
<
0.05）。
Logistic
回归分析结果显示，lncRNA XIST、miR-126-3p、淋巴结转移、TNM分期是NSCLC患者术后复发的影响因素（
P
<
0.05）；血清lncRNA XIST、miR-126-3p及二者联合预测NSCLC患者术后复发的曲线下面积（
AUC
）分别为0.750、0.886、0.933，二者联合预测优于单独预测（
Z
二者联合
vs.
lncRNA XIST
=4.076、
Z
二者联合
vs.
miR-126-3p
=2.065，
P
<
0.05）。
结论
2
NSCLC患者血清lncRNA XIST表达升高，miR-126-3p表达降低，两者对于患者根治术后复发具有一定的预测价值。
Abstract
Objective
2
To investigate the expression of serum long non-coding RNA X-inactive specific transcript （lncRNA XIST） and microRNA-126-3p （miR-126-3p） in patients with non-small cell lung cancer （NSCLC），and their relationship with recurrence after radical surgery.
Methods
2
A total of 108 NSCLC patients who underwent radical surgery and were admitted to General Hospital of Southern Theater Command of the People's Liberation Army of China from February 2019 to October 2020 were selected as the NSCLC group，and 52 healthy volunteers who underwent physical examination in this hospital were selected as the control group. qRT-PCR method was used to detect the relative expression levels of serum lncRNA XIST and miR-126-3p.
Pearson
method was used to analyze the correlation between serum lncRNA XIST and miR-126-3p expression.
Logistic
regression analysis was used to analyze the influencing factors of postoperative recurrence in NSCLC patients.
Receiver operating characteristic
（
ROC
） curve was used to evaluate the predictive value of serum lncRNA XIST and miR-126-3p on postoperative recurrence in patients.
Results
2
The expression level of serum lncRNA XIS
T in the NSCLC group was higher than that in the control group （
P
<
0.05），while the expression level of miR-126-3p was lower than that in the control group （
P
<
0.05）. The expression levels of serum lncRNA XIST and miR-126-3p were related to TNM staging and lymph node metastasis （
P
<
0.05）. Bioinformatics website predicted that there was a targeted binding site between lncRNA XIST and miR-126-3p，and serum lncRNA XIST was negatively correlated with miR-126-3p expression （
r
=-0.579，
P
<
0.05）. Compared with the non-recurrent group，the expression level of serum lncRNA XIST in the recurrent group increased （
P
<
0.05），while the expression level of miR-126-3p in the recurrent group decreased （
P
<
0.05）.
Logistic
regression analysis results showed that lncRNA XIST，miR-126-3p，lymph node metastasis，and TNM staging were the influencing factors for postoperative recurrence in NSCLC patients （
P
<
0.05）. The
area under the curve
（
AUC
） of serum lncRNA XIST，miR-126-3p，and the combination of the two in prediction of postoperative recurrence in NSCLC patients were 0.750，0.886，and 0.933，respectively，the combined prediction of the two was superior to individual prediction （
Z
combination
vs
. lncRNA XIST
=4.076，
Z
combination
vs
.miR-126-3p
=2.065，
P
<
0.05）.
Conclusion
2
The expression of serum lncRNA XIST is increased and miR-126-3p is decreased in NSCLC patients，both of which have certain predictive value for recurrence after radical surgery in patients.
关键词
非小细胞肺癌lncRNA XISTmiRNA-126-3p根治术复发
Keywords
non-small cell lung cancerlncRNA XISTmiRNA-126-3pradical surgeryrecurrence
references
Chen P，Liu Y，Wen Y，et al.Non-small cell lung cancer in China［J］.Cancer Commun （Lond），2022，42（10）：937-970.doi：10.1002/cac2. 12359http://dx.doi.org/10.1002/cac2.12359.
Li M，Hu M，Jiang L，et al.Trends in cancer incidence and potential associated factors in China［J］.JAMA Netw Open，2024，7（10）：e2440381.doi：10.1001/jamanetworkopen.2024.40381http://dx.doi.org/10.1001/jamanetworkopen.2024.40381.
Xia C，Dong X，Li H，et al.Cancer statistics in China and United States，2022：profiles，trends，and determinants［J］.Chin Med J （Engl），2022，135（5）：584-590.doi： 10.1097/CM9.0000000000002108http://dx.doi.org/10.1097/CM9.0000000000002108.
Ganti AK，Klein AB，Cotarla I，et al.Update of incidence，prevalence，survival，and initial treatment in patients with non-small cell lung cancer in the US［J］.JAMA Oncol，2021，7（12）：1824-1832.doi：10.1001/jamaoncol.2021.4932http://dx.doi.org/10.1001/jamaoncol.2021.4932.
Liao Y，Cheng S，Xiang J，et al.lncRNA CCHE1 increased proliferation，metastasis and invasion of non-small lung cancer cells and predicted poor survival in non-small lung cancer patients［J］.Eur Rev Med Pharmacol Sci，2018，22（6）：1686-1692.doi：10.26355/eurrev_201803_14581http://dx.doi.org/10.26355/eurrev_201803_14581.
Xing C，Sun SG，Yue ZQ，et al.Role of lncRNA LUCAT1 in cancer［J］.Biomed Pharmacother，2021，134：111158.doi：10.1016/j.biopha. 2020.111158http://dx.doi.org/10.1016/j.biopha.2020.111158.
Cao H，Wahlestedt C，Kapranov P.Strategies to annotate and characterize long noncoding RNAs：advantages and pitfalls［J］.Trends Genet，2018，34（9）：704-721.doi：10.1016/j.tig.2018.06.002http://dx.doi.org/10.1016/j.tig.2018.06.002
Hu J，Huang H，Xi Z，et al.LncRNA SEMA3B-AS1 inhibits breast cancer progression by targeting miR-3940/KLLN axis［J］.Cell Death Dis，2022，13（9）：800.doi：10.1038/s41419-022-05189-7http://dx.doi.org/10.1038/s41419-022-05189-7.
Soliman SE，Abdelaleem AH，Alhanafy AM，et al.Circulating miR-21-5p and miR-126-3p：diagnostic，prognostic value，and multivariate analysis in non-small-cell lung cancer［J］.Mol Biol Rep，2021，48（3）：2543-2552.doi：10.1007/s11033-021-06302-3http://dx.doi.org/10.1007/s11033-021-06302-3.
Gupta S，Silveira DA，Hashimoto RF，et al.A boolean model of the proliferative role of the lncRNA XIST in non-small cell lung cancer cells［J］.Biology （Basel），2022，11（4）：480.doi：10.3390/biology 11040480http://dx.doi.org/10.3390/biology11040480.
Zhou Q，Hu W，Zhu W，et al.Long non coding RNA XIST as a prognostic cancer marker： a meta-analysis［J］.Clin Chim Acta，2018，482：1-7.doi：10.1016/j.cca.2018.03.016http://dx.doi.org/10.1016/j.cca.2018.03.016
王丽.中国原发性肺癌诊疗规范（2015年版）［J］.中华肿瘤杂志，2016，37（7）：433-436.doi：10.13683/j.wph.2016.07.001http://dx.doi.org/10.13683/j.wph.2016.07.001.
钱行.血清SCC-Ag、CEA水平与非小细胞肺癌患者靶向治疗预后的相关性分析［J］.现代诊断与治疗，2021，32（20）：3297-3298.
许永红，李峰，陈俊辉，等.非小细胞肺癌常见中医证型与血清SCC、CYFRA21-1、NSE水平相关性研究［J］.四川中医，2023，41（8）：87-91.
曾媛，卢孝清，张妮，等.MET、TXA2在非小细胞肺癌组织中的表达及临床意义［J］.临床肺科杂志，2020，25（9）：1399-1404.
舒春露，朱大兴，周清华.非小细胞肺癌新辅助免疫治疗生物标志物研究进展［J］.中国肺癌杂志，2022，25（2）：130-136.doi：10.3779/j.issn.1009-3419.2022.102.04http://dx.doi.org/10.3779/j.issn.1009-3419.2022.102.04.
Wang W，Min L，Qiu X，et al.Biological function of long non-coding RNA （LncRNA） Xist［J］.Front Cell Dev Biol，2021，9：645647.doi：10.3389/fcell.2021.645647http://dx.doi.org/10.3389/fcell.2021.645647
Xu X，Zhou X，Chen Z，et al.Silencing of lncRNA XIST inhibits non-small cell lung cancer growth and promotes chemosensitivity to cisplatin［J］.Aging （Albany NY），2020，12（6）：4711-4726.doi：10.18632/aging.102673http://dx.doi.org/10.18632/aging.102673.
Li C，Wan L，Liu Z，et al.Long non-coding RNA XIST promotes TGF-β-
induced epithelial-mesenchymal transition by regulating miR-367/141-ZEB2 axis in non-small-cell lung cancer［J］.Cancer Lett，2018，418：185-195.doi：10.1016/j.canlet.2018.01.036http://dx.doi.org/10.1016/j.canlet.2018.01.036.
Wang X，Zhang G，Cheng Z，et al.Knockdown of LncRNA-XIST suppresses proliferation and TGF-β1-induced EMT in NSCLC through the Notch-1 pathway by regulation of miR-137［J］.Genet Test Mol Biomarkers，2018，22（6）：333-342.doi：10.1089/gtmb.2018. 0026http://dx.doi.org/10.1089/gtmb.2018.0026.
Chen J，Ding C，Yang X，et al.BMSCs-derived exosomal miR-126-3p inhibits the viability of NSCLC cells by targeting PTPN9［J］.J BUON，2021，26（5）：1832-1841.
Huang W，Chen Q，Dai J，et al.Long noncoding TMPO antisense RNA 1 promotes hepatocellular carcinoma proliferation and epithelial-mesenchymal transition by targeting the microRNA-126-3p/LRP6/ β-catenin axis［J］.Ann Transl Med，2021，9（22）：1679.doi：10.21037/atm-21-5593http://dx.doi.org/10.21037/atm-21-5593.
Fang H，Yang L，Fan Y，et al.Upregulation of tissue long noncoding RNA X inactive specific transcript predicts poor postoperative survival in patients with non-small cell lung cancer［J］.Medicine （Baltimore），2020，99（50）：e21789.doi：10.1097/MD.000000000 0021789http://dx.doi.org/10.1097/MD.0000000000021789.
Ulivi P，Petracci E，Marisi G，et al.Prognostic role of circulating miRNAs in early-stage non-small cell lung cancer［J］.J Clin Med，2019，8（2）：131.doi：10.3390/jcm8020131http://dx.doi.org/10.3390/jcm8020131.
0
浏览量
0
下载量
0
CSCD
- 网络PDF下载
- WORD下载
- Meta-XML下载
- BibTeX下载
- Endnote下载
- RefMan 下载
- NoteExpress下载
- NoteFirst下载
关联资源
相关文章
相关作者
相关机构
- 技术支持由北京北大方正电子有限公司提供 京ICP备09064830号-19
京公网安备11010802024621 - 本系统建议在Chrome、 IE9+ 以上版本浏览器阅读本站内容，360浏览器请切换至极速模式
- Cookies帮助我们提供服务并提供个性化体验。使用本网站，即表示您同意我们使用Cookies
