LPS对小鼠肺组织及RAW264.7细胞Akt蛋白表达及磷酸化的调控

尹华华; 夏浩; 程晓刚; 朱京慈; 闫红; 刘道城; 李磊

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LPS对小鼠肺组织及RAW264.7细胞Akt蛋白表达及磷酸化的调控

论著 | 更新时间：2025-01-15

- LPS对小鼠肺组织及RAW264.7细胞Akt蛋白表达及磷酸化的调控
- Effect of LPS on Akt expression and phosphorylation in mouse lung tissue and RAW264.7 cells
- 陆军军医大学学报 2007年29卷第3期页码：188-190
- 作者机构：
  
  1. 第三军医大学：大坪医院野战外科研究所第一研究室，创伤烧伤与复合伤国家重点实验室，护理系
  2. 成都医学院病原学教研室
- 作者简介：
- 基金信息：
- DOI：
  中图分类号： R329.26;R631.102;R392.11
- 纸质出版日期：2007，
  
  网络出版日期：2007-02-15，
- 稿件说明：
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尹华华, 夏浩, 程晓刚, 等. LPS对小鼠肺组织及RAW264.7细胞Akt蛋白表达及磷酸化的调控[J]. 陆军军医大学学报, 2007,29(3):188-190.

YIN HUA-HUA, XIA HAO, CHENG XIAO-GANG, et al. Effect of LPS on Akt expression and phosphorylation in mouse lung tissue and RAW264.7 cells. [J]. Journal of army medical university, 2007, 29(3): 188-190.
尹华华, 夏浩, 程晓刚, 等. LPS对小鼠肺组织及RAW264.7细胞Akt蛋白表达及磷酸化的调控[J]. 陆军军医大学学报, 2007,29(3):188-190. DOI：

YIN HUA-HUA, XIA HAO, CHENG XIAO-GANG, et al. Effect of LPS on Akt expression and phosphorylation in mouse lung tissue and RAW264.7 cells. [J]. Journal of army medical university, 2007, 29(3): 188-190. DOI：

摘要

目的探讨脂多糖 (lipopolysaccharide

LPS)在体内外对Akt蛋白表达和磷酸化水平的影响。方法用不同剂量LPS攻击RAW264.7细胞，检测不同时相点细胞Akt蛋白表达和磷酸化水平。小鼠腹腔注射LPS 6 h 后检测肺组织Akt蛋白表达和磷酸化水平。结果不同剂量LPS攻击RAW264.7细胞，细胞Akt蛋白表达没有显著差异(

>0.05)，磷酸化水平增加。而内毒素血症小鼠肺组织Akt蛋白表达没有显著变化(

>0.05)，磷酸化水平减低。结论 LPS在体内外对Akt蛋白磷酸化作用不同，体外Akt蛋白磷酸化水平增高，但在内毒素血症小鼠体内出现Akt蛋白磷酸化水平异常减低。

Abstract

Objective To explore the effect of LPS on the expression and phosphorylation of Akt

a PTEN/PI3k signal transduction downstream molecule

in vivo

and

in vitro

. Methods RAW264.7 cells were attacked with LPS at different concentrations. The Akt protein expression and phosphorylation level were detected by Western blotting at 8

48 h after attack. Six h after receiving 20 mg/kg LPS intra-peritoneal injection

the mice were killed and their lung tissues were resected for Akt protein expression and phosphorylation. Results The Akt protein expression of RAW264.7 cells showed no obvious difference after LPS attack at different doses

but their phosphorylation l

evel was increased obviously with the increase of the doses. Whereas the phosphorylation level of Akt protein was decreased in the lung tissue of mice 6 h after LPS attack. Conclusion The phosphorylation level of Akt protein is different after LPS attack

in vitro

and

in vivo

. The Akt protein phosphorylation is increased obviously

in vitro

but decreased in mice model of endotoxemia.

关键词

脂多糖AKT磷酸化内毒素血症

Keywords

LPSAKTphosphorylationendotoxemia

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