Objective     To study the apoptosis-inducing effects and mechanisms of influenza virus A H

3

N

2

on a self-established endometrial adenocarcinoma cell line JEC.      Methods     JEC cells were infected with different concentrations of H

3

N

2

the apoptosis were detected using the HE staining

DNA agarose gel electrophoresis and flow cytometry. The expressions of Fas

FasL and TGF-β were examined by immunocytochemical staining.      Results     After infection

the JEC cells showed the morpholog

ical apoptosis; DNA agarose electrophoresis demonstrated a ladder-like pattern of DNA fragments; FITC/PI stained FCM showed the apoptotic rate of JEC had been decreased along with the prolonging of infected time and elevated with the increase of the virus concentrations. Immunocytochemical staining showed that enhanced expression of Fas

attenuated expression of TGF-β

and no expression of FasL after H

3

N

2

infection.      Conclusion     Influenza virus A H

3

N

2

can induce JEC cells to apoptosis in time- and concentration- dependent manners

which may be related with the expressions of Fas and TGF-β.