Objectives     To observe the effects of glucose at different concentrations on the expression of PPARδ in the rat insulinoma INS-1 cells so as to illustrate the function of PPARδ in the cross-talk of lipid metabolism with glucose metabolism in pancreatic cells.      Methods     After cultured in RPMI1640 media containing 3

11 and 20 mmol/L glucose respectively for 1 h

the cells were used to measure the content of intracellular triglyceride. The RNA and nuclear protein of INS-1 cells were extracted

and then the expression of PPARδ mRNA was detected by semi-quantity RT-PCR and the PPARδ protein was analyzed by Western blotting.      Results     With the increment of the concentrations of glucose

the content of intracellular triglyceride in the cultured cells was elevated

while the expressions of PPARδ mRNA and protein were both down-regulated.      Conclusion     Glucose promotes triglyceride deposition in INS-1 cells

and the down-regulation of PPARδ presumably contributes to the lipid deposition in INS-1 cells during hyperglycemic states.