Objective     To establish a quality analysis method for the compound chlorhexidine gargle

and study its inhibitory effect of anti-oral aerobic bacteria in

vitro

.      Methods     The concentrations of 3 ingredients in the compound chlorhexidine gargle were determined by high-performance liquid chromatography (HPLC)

S. aureus

S. epidermidis

，

Ps

.

aeruginosa

and

E. coli

were chosen as the experimental bacteria. Minimal inhibitory concentration (MIC) was measured by agar dilution test.      Results     A good linear relationship was observed when chlorhexidine acetate

dyclonine hydrochloride and dexamethason

e were respectively in a range of 8-40

10-50

and 10-50 μg/ml. Their correlation coefficients were 0.999 2

0.999 4 and 0.999 9 respectively. The compound chlorhexidine gargle showed better bacteriostatic effects on above-mentioned bacteria.      Conclusion     The compound chlorhexidine gargle was a better oral preparation and might be an effective agent in treating

S. aureus

S. epidermidis

，

Ps. aeruginosa

and

E. coli

caused diseases.