Objective     To establish two-dimensional electrophoresis profiles of laryngeal squamous carcinoma tissue with high resolution and reproducibility.      Methods     The total protein of well-differentiated or poorly-differentiated laryngeal cancer tissue was separated by means of immobilized pH gradient-based two-dimensional gel electrophoresis (2-DE). The differential expression protein was analyzed by using ImageMaster 2D analysis software.      Results     We obtained fine resolution

reproducible 2-DE patterns of well-differentiated and poorly-differentiated laryngeal squamous carcinoma. Differential expression protein spots were defined in 2-DE gels. Thirteen protein spots were preliminarily identified

among which seven proteins were upregulated in well-differentiated laryngeal cancer tissue

six were downregulated.      Conclusion     The expression map of proteomics was established for well-differentiated and poorly-differentiated laryngeal cancer

which was of significant difference between them. Laryngeal cancer proteome might reflect the underlying pathological state.