Objective    To investigate the differential expressions of microRNAs in IgA nephropathy patients by using microRNAs array.     Methods    Samples of 11 patients with IgA nephropathy were collected by biopsy (IgA nephropathy group)

and 3 renal cortexes of eumorphism patients as normal control group. Total RNA of every group was extracted using Trizol according to the manufacturer’s instruction. MicroRNAs were isolated from total RNA and significant differential expression microRNAs were screened by microRNAs array. The reliability of the results of microRNAs array was validated by Real-time PCR.     Results    In IgA nephropathy

there were 66 microRNAs significant differential expression of which 35 up-regulated and 31 down-regulated. hsa-miR-637 and hsa-miR-492 were selected for relative quantification by Real-time PCR. It showed that the results of both methods had no significant deviation and the results of microRNAs array was reliable.     Conclusion    IgA nephropathy is a classic multiple factors

polygenic disease and until now there is no mechanism which can identify it perfectly. Our data indicate that microRNAs are significant differential expression in IgA nephropathy. The lucubrated of microRNAs in IgA nephropathy may help to diagnosis

treat and prevent IgA nephropathy.