Objective    To study the correlation between rotavirus nonstructural protein 1 (NSP1) and species restriction of the virus and explore the role of NSP1 in the species restriction.     Methods    NSP1 C-terminal sequences

its whole amino acid sequences from UniProt Database

and its 3′ noncoding sequences from GenBank were collected

and intraspecies and interspecies sequence alignment and phylogenetic tree analysis were performed by DNAStar (Lasergene)7.1.     Results    The diversity of NSP1 between species and rotavirus species restriction hosted a high degree of consistency. NSP1 C-terminal se

quence

NSP1 gene 3′ noncoding sequence and NSP1 whole sequence shared a high intraspecies identity

which was higher than the identity between species (

P

<

0.01).     Conclusion    NSP1 and rotavirus species restriction are closely related. Interaction between NSP1 and host specificity interferon regulatory factor 3 (IRF3) may be related to the rotavirus species restriction

and their binding region and the 3′  noncoding sequence of NSP1 gene may be also related to RV species restriction.