Objective  To investigate the influence of NO mixture (L-arginine

apocynin and sodium nitroferricyanide) on wound healing of diabetes mellitus (DM) mice. Methods  Fifty adult male Kunming mice were induced by streptozotocin to establish type I DM model. They were then randomly divided into control group

L-arginine group (150 g/L)

apocynin group (1×10

-4

mol/L)

sodium nitroferricyanide group (0.1 mmol/L) and NO mixture group (above components were mixed with equal ratio). Full-thickness skin wound was made and injected with corresponding drugs

of 0.15 ml once every 2 days. The wounds were digitally photographed to calculate the percentage of wound closure using computer image analysis software in 1

3

5

7 and 10 d post-injury. The density of fibroblasts

the content of collagenous fibers and the neovascularization in the wound samples were measured with the aid of HE staining. Results  From the third day post-injury

the wound healing rate of NO mixture group was significantly higher than that of all the other groups (

P

<

0.01). Compared with control group

the density of fibroblasts

the content of collagenous fibers and the neovascularization were all increased in medicine-injected group  (

P

<

0.05，

P

<

0.01). However

the difference between NO mixture group and the control group was the most significant one among all the groups. Conclusion  NO mixture effectively enhances the wound healing of DM mice

and its promotion is much more significant than that of L-arginine

apocynin and sodium nitroferricyanide used alone.