Objective  To determine the antibacterial effect of the oil extracted from

Zingiber corallinum Hance 

on inhibiting drug-resistant bacteria and acute toxicity. Methods  Susceptibility test of paper disk method was used to investigate the effect of

Zingiber corallinum Hance

oil inhibiting drug-resistant bacteria. The oral gavage experiment of fasting mice was used to observe the effect of the oil’s acute toxicity and to determine half lethal dose (LD

50

). Results 

Zingiber corallinum Hance

oil excerted obvious inhibitor

y effect on gram-negative drug-resistance bacteria （

P

<

0.05）

such as

Escherichia coli

Klebsiella pneumoniae

Enterobacter cloacae

and

Acinetobacter baumannii

but the inhibitory effect on

Pseudomonas aeruginosa

was not obvious.

Zingiber corallinum Hance

oil also had inhibitory effect on gram-positive drug-resistant bacteria （

P

<

0.05）

such as

Staphylococcus aureus

Staphylococcus epidermidis

and

hemolytic Staphylococcus

. The oil containing 79% terpinene-4-alcohol showed better bacteriostatic action than that of 34% content （

P

<

0.05）. The LD

50

of

Zingiber corallinum Hance

oil is 1 790.427 mg/kg. Conclusion  As a low toxicity drug

Zingiber corallinum Hance

oil obviously inhibits drug-resistant bacteria

and terpinene-4-alcohol is an important inhibitory competent content of the oil.