RNA干扰沉默STIM1表达对低氧下大鼠肺动脉平滑肌细胞增殖的影响

您当前的位置：

首页 >

文章列表页 >

RNA干扰沉默STIM1表达对低氧下大鼠肺动脉平滑肌细胞增殖的影响

论著 | 更新时间：2025-01-15

|

- RNA干扰沉默STIM1表达对低氧下大鼠肺动脉平滑肌细胞增殖的影响
- Effect of RNA interference targeting STIM1 gene on proliferation of hypoxic pulmonary artery smooth muscle cells in rat
- 陆军军医大学学报 2011年33卷第5期页码：437-440
- 作者机构：
  
  第三军医大学：高原军事医学系病理生理与高原生理学教研室，教育部高原医学重点实验室，全军高原生理与高原病研究重点实验室，高原疾病学教研室
- 作者简介：
- 基金信息：
- DOI：
  中图分类号： R322.121;R394.2;R364.4
- 纸质出版日期：2011，
  
  网络出版日期：2011-03-15，
- 稿件说明：
移动端阅览
后显华, 陈建, 罗勇军, 等. RNA干扰沉默STIM1表达对低氧下大鼠肺动脉平滑肌细胞增殖的影响[J]. 陆军军医大学学报, 2011,33(5):437-440.

HOU XIANHUA, CHEN JIAN, LUO YONGJUN, et al. Effect of RNA interference targeting STIM1 gene on proliferation of hypoxic pulmonary artery smooth muscle cells in rat. [J]. Journal of army medical university, 2011, 33(5): 437-440.
后显华, 陈建, 罗勇军, 等. RNA干扰沉默STIM1表达对低氧下大鼠肺动脉平滑肌细胞增殖的影响[J]. 陆军军医大学学报, 2011,33(5):437-440. DOI：

HOU XIANHUA, CHEN JIAN, LUO YONGJUN, et al. Effect of RNA interference targeting STIM1 gene on proliferation of hypoxic pulmonary artery smooth muscle cells in rat. [J]. Journal of army medical university, 2011, 33(5): 437-440. DOI：

摘要

目的应用RNA干扰抑制肺动脉平滑肌细胞(pulmonary aretery smooth muscle cells，PASMCs)基质交感分子1(stromal interaction molecule 1，STIM1)基因表达，探讨STIM1表达抑制后对PASMCs缺氧增殖反应的影响。方法采用离体培养大鼠肺动脉平滑肌细胞，设对照组、阴性siRNA组（SiNT）及阳性siRNA组（SiSTIM1），低氧（3%O

2

）培养24 h，Western blot法检测PASMCs中STIM1基因表达变化，MTT比色法及[

3

H

]

-TdR掺入法检测PASMCs的增殖情况，流式细胞仪检测PASMCs的细胞周期变化。结果 Western blot检测SiSTIM1组STIM1蛋白相对灰度值为(0.355±0.008)，显著低于对照组(0.624±0.017)和SiNT组(0.618±0.021) (

P

<

0.05)。MTT比色实验及[

3

H

]

-TdR掺入实验显示低氧培养24 h 后，对照组和SiNT组PASMCs较常氧组增殖显著(

P

<

0.05)，而SiSTIM1组PASMCs增殖活性较对照组和SiNT组显著降低(

P

<

0.05)。流式细胞仪检测结果表明低氧培养24 h后，对照组和SiNT组PASMCs G

0

/G

1

期细胞比例较常氧组显著降低 (

P

<

0.05)，而SiSTIM1组G

0

/G

1

期细胞比例则显著高于对照组和SiNT组(

P

<

0.05)。结论 RNA干扰介导STIM1基因沉默可明显抑制低氧下大鼠肺动脉平滑肌细胞增殖。

Abstract

Objective To inhibit the expression of stromal interaction molecule 1 (STIM1) gene of pulmonary artery smooth muscle cells (PASMCs) through RNA interference

and to explore the proliferation ch

ange of PASMCs under hypoxia condition after gene silencing. Methods

In vitro

cultured PASMCs were divided into a blank control group

a negative control siRNA group (SiNT) and a positive siRNA group (SiSTIM1) that were cultured in hypoxia condition (3% O

2

) for 24 h

as well as a normal group that was cultured in normal O

2

condition (21% O

2

) for 24 h. Western blotting

MTT assay and [

3

H

]

-TdR incorporation assay

and flow cytometry were employed to test STIM1 gene expression

cell proliferation

and cell cycle of PASMCs

respectively. Results Western blotting results showed that the relative grey value of STIM1 protein in the SiSTIM1 group (0.355±0.008) was remarkably lower than that in the control group (0.624±0.017) and the SiNT group (0.618±0.021) (

P

<

0.05). MTT assay and [

3

H

]

-TdR incorporation assay exhibited that

after 24 h hypoxic culture

the PASMCs in the blank control group and the SiNT group proliferated significantly as compared with those in the normal group (

P

<

0.05) and the SiSTIM1 group (

P

<

0.05). Flow cytometry results discovered that

after hypoxic culture for 24 h

the percentages of PASMCs in G

0

/G

1

phase in the blank control group and the SiNT group were remarkably lower than those in the normal group (

P<

0.05) and the SiSTIM1 group (

P

<

0.05). Conclusion RNA interference-mediated STIM1 gene silencing can significantly inhibit the proliferation of PASMCs under hypoxia.

关键词

基质交感分子1RNA干扰肺动脉平滑肌细胞缺氧细胞增殖

Keywords

references

0

浏览量

0

下载量

0

CSCD

文章被引用时，请邮件提醒。

提交

工具集

关联资源

相关文章

PDGF在缺氧肺动脉平滑肌细胞增殖中的作用及其机理初探

大鼠肺动脉平滑肌细胞缺氧后STAT3活性变化对细胞癌基因c-jun mRNA表达及细胞增殖的影响

缺氧对肺动脉内皮细胞PDGF─A、─B链及其受体α、β亚基基因表达的影响

一氧化碳对大鼠肺动脉平滑肌细胞[Ca2+]、cAMP、cGMP的作用

血管平滑肌细胞受低浓度一氧化碳作用后迁移的变化

相关作者

刘健

罗德成

王培勇

孙秉庸

白莉

余祖滨

钱频

钱桂生

相关机构

第三军医大学基础医学部病理生理学教研室

第三军医大学新桥医院全军呼吸内科研究所,全军呼吸病研究重点实验室

第三军医大学新桥医院胸外科

第三军医大学新桥医院超声诊断科

第三军医大学基础部病理生理学教研室

⁰