Objective      To determine the effect of two different silylation reagents on surface modification of polydimethylsiloxane (PDMS) polymer microfluidic cell chip and investigate the cell capture/screen initial application of the modified microfluidic chip.       Methods      Hydrophilicity of samples treated by silylation reagents was measured by contact angle to screen silane reagent and determine optimal concentration. The results were verified by non-specific cell capture experiments. Then immobilized antibody was used to establish functional cell chip for further study on cell capture.       Results      In this study

both two silylation reagents reduced the non-specific adsorption. GPTMS at 2%(volume concentration) obtained a smallest contact angle and resulted in non-specific adsorption of cells reduced to 5.3%. The capture rate was increased to 53.96% after antibody immobilization.       Conclusion      An effective silylation reagent GPTMS is screened and its best concentration for surface modification of PDMS chip is 2%. In this condition

PDMS is hydrophilic and has a lower adsorption for non-specific cells. This modification is benefit to preparation of functioned cell capture chips.