Objective      To study the correlation between inhibitory effect of resveratrol on proliferation of A549 human lung adenocarcinoma cells and IGF-ⅠR.       Methods      Proliferation of A549 human lung andenocarcinoma cells was detected by methyl thiazolyl tetrazolium (MTT) assay. Expression level of IGF-ⅠR mRNA and protein was measured by RT-PCR and Western blotting

respectively.       Results      IGF-Ⅰ at the concentration of 2.5 to 15 nmol/L significantly increased the proliferation of A549 human lung andenocarcinoma cells (

P

<

0.05) and resvera

trol at the concentrations of 12.5

25.0  and 50 μmol/L remarkably inhibited the proliferation of A549 human lung andenocarcinoma cells (

P

<

0.05). Combined resveratrol (6.25 to 50 μmol/L) and IGF-Ⅰ (10 nmol/L) could inhibit the IGF-ⅠR-induced proliferation of A549 human lung andenocarcinoma cells

especially at the concentrations of 25.0  and 50.0 μmol/L (

P

<

0.05). Resveratrol could effectively down-regulate the expression of IGF-ⅠR mRNA and protein.       Conclusion      Resveratrol can effectively inhibit the IGF-ⅠR-induced proliferation of A549 human lung adenocarcinoma cells by down-regulating the expression of IGF-ⅠR mRNA and protein.