肺结核患者外周血中CD4+CD25+FoxP3+调节T细胞增多并抑制抗结核免疫

胡良安; 李岱容; 罗永艾; 杨相梅; 黄习臣

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肺结核患者外周血中CD4⁺CD25⁺FoxP3⁺调节T细胞增多并抑制抗结核免疫

论著 | 更新时间：2025-01-15

- 肺结核患者外周血中CD4⁺CD25⁺FoxP3⁺调节T细胞增多并抑制抗结核免疫
- Peripheral blood CD4⁺CD25⁺FoxP3⁺regulatory T cells in active pulmonary tuberculosis patients
- 陆军军医大学学报 2011年33卷第20期页码：2124-2127
- 作者机构：
  
  重庆医科大学附属第一医院肺科
- 作者简介：
- 基金信息：
- DOI：
  中图分类号： R521.02;R392.11;R392.32
- 纸质出版日期：2011，
  
  网络出版日期：2011-10-30，
- 稿件说明：
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胡良安, 李岱容, 罗永艾, 等. 肺结核患者外周血中CD4⁺CD25⁺FoxP3⁺调节T细胞增多并抑制抗结核免疫[J]. 陆军军医大学学报, 2011,33(20):2124-2127.

HU LIANG’AN, LI DAIRONG, LUO YONG’AI, et al. Peripheral blood CD4⁺CD25⁺FoxP3⁺regulatory T cells in active pulmonary tuberculosis patients. [J]. Journal of army medical university, 2011, 33(20): 2124-2127.
胡良安, 李岱容, 罗永艾, 等. 肺结核患者外周血中CD4⁺CD25⁺FoxP3⁺调节T细胞增多并抑制抗结核免疫[J]. 陆军军医大学学报, 2011,33(20):2124-2127. DOI：

HU LIANG’AN, LI DAIRONG, LUO YONG’AI, et al. Peripheral blood CD4⁺CD25⁺FoxP3⁺regulatory T cells in active pulmonary tuberculosis patients. [J]. Journal of army medical university, 2011, 33(20): 2124-2127. DOI：

摘要

目的研究肺结核患者外周血中CD4

CD25

FoxP3

调节T细胞是否增多，这些调节T细胞是否抑制结核的特异细胞免疫反应。方法使用细胞分离、流式细胞分析及细胞增殖及增殖抑制等实验方法，对20例肺结核患者及17例健康人群外周血中CD4

CD25

FoxP3

调节T细胞的数量及免疫学特征作研究。结果肺结核患者外周血中CD4

CD25

FoxP3

调节T细胞数占CD4

细胞总数的比例显著高于健康人群(

0.01)。在体外，肺结核患者外周血单个核细胞在BCG及ESAT-6的刺激下增殖能力比健康对照人群明显增强(

0.01)

产生的γ-干扰素比健康人群也明显增强(

0.05)；清除调节T细胞后的肺结核患者外周血单个核细胞对BCG刺激下产生γ-干扰素及白介素-10比没有清除时显著增强(

0.05)；从肺结核患者外周血分离CD4

CD25

调节T细胞和CD4

CD25

细胞

CD4

CD25

细胞对BCG及ESAT-6刺激产生γ-干扰素和白介素-10的能力因CD4

CD25

调节T细胞加入后而显著减弱(

0.05)。结论肺结核患者CD4

CD25

FoxP3

调节T细胞增多，通过抑制结核患者细胞免疫反应，参与肺结核的发病。

Abstract

Objective To determine whether CD4

CD25

FoxP3

regulatory T cells(Treg) are increased in p

atients with active pulmonary tuberculosis(TB) and whether they suppress specific cellular immune responses. Methods The frequency and the immune function of circulating regulatory T cells were compared in 20 untreated patients with active pulmonary TB and 17 healthy matched control by using cell separation

flow cytometry analysis

proliferation assays and cytokines determination. Results The proportion of CD4

CD25

FoxP3

regulatory T cells within CD4

T cells was significantly increased in the blood of active pulmonary TB patients than heathy control subjects (

0.01). Responsing to both Bacille Calmette Guerin (BCG) and early secretory antigenic target-6 (ESAT-6)

the peripheral blood mononuclear cells (PBMCs) of pulmonary TB patient had significantly higher cellular proliferation compared to the PBMCs from healthy donors (both

0.01)

and had higher IFN-γ production(both

0.05). PBMCs from pulmonary TB patient had significantly higher BCG-induced IFN-γ and IL-10 production after CD4

CD25

regulatory T cells depletion than before (both

0.05). CD4

CD25

cells isolated from TB patients had significantly lower BCG-stimulated and ESAT-6-stimulated IFN-γ production and IL-10 production with CD4

CD25

T cells from TB patients than in the cells without CD4

CD25

T cells (both

0.05). Conclusion CD4

CD25

FoxP3

regulatory T cells are expanded in patients with active pulmonary TB

and therefore contribute to the pathogensis of human TB by suppressing specific

Mycobacterium tuberculosis

cellular immune responses.

关键词

T淋巴细胞CD4+CD25+FoxP3+调节T细胞肺结核分枝杆菌结核免疫

Keywords

T-lymphocytesCD4+CD25+FoxP3+regulatory T cellspulmonary tuberculosisMycobacterium tuberculosisimmunity

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