Objective      To explore the effect of silencing heparanase gene by RNA short hairpin on the proliferation and invasion in human ovarian cancer cell line SKOV3.       Methods      After shRNA lentiviral vectors targeting heparanase gene was constructed

they were transfected into SKOV3 cells. Experimental transfection groups included the interference sequence

s HPA shRNA-1 group

HPA shRNA-2

and  HPA shRNA-3 group

and the untransfected group amd blank transfection group served as control. After transfection

the interference efficiency was observed though fluorescence quantitative PCR and Western blotting to detect the expression of heparanase at mRNA and protein levels. Flow cytometry was employed to test cell cycle changes

cell counting kit-8 (CCK-8) for the detection of cell proliferation

and matrix gel invasion assays for the detection of cell invasion ability.       Results      After shRNA lentiviral vectors were transfected into SKOV3 cells

HPA expression was significantly decreased at mRNA and protein levels in the HPA-shRNA-1 sequence and the HPA-shRNA-2 sequence groups (

P

<

0.05)

whereas the expression levels of HPA-shRNA-3 sequence group was not decreased

indicating it was an ineffective sequence. In the SKOV3 cells transfected with effective sequences HPA-shRNA-1 and HPA-shRNA-2

the cell percentage of G

1

phase was decreased

the proliferation (OD index) was significantly decreased and transmembrane cell number was increasingly decreased. There was a significantly difference between the experimental groups and untransfected and blank transfection groups respectively (

P

<

0.05).       Conclusion      Silencing HPA by RNA interference inhibits heparanase expression

and suppresses cell proliferation and invasion efficiently

which may be correlated with the downregulation of HPA gene and protein.