Objective      To construct a rapid and highly sensitive detection system based on gold nanoparticle probes and protein chip.       Methods      The capture antibody (capture for target antigen) was combined to the aldehyde modified glass

and the nanoparticle probes were prepared using detection antibody. Then

the sandwich structures (protein chip-the target protein-gold nanoparticle probes) were created through the interaction between the antibody and antigen

and dyed by the gold deposition solution. The results were observed with the naked eye or by microscopy.       Results      For this protein detection system

CEA and CYFRA21-1 could be detected as low as 45 pg/mL and 90 pg/mL respectively in 1 h. The sensitivity was improved greatly

compared with the traditional ELISA method. Using this system

the lung cancer patients and normal people detection results were consistent with clinical used method (electrochemical analysis).       Conclusion      This system for CEA and CYFRA21-1 detection is an easy

rapid

convenient method with high sensitivity and multiple detection

which might have an important value and great application prospect in the clinical protein detection.