载异烟肼、利福平白蛋白纳米粒的制备及其体外释药研究

马荣; 陈振; 张惠勇; 杨小英; 王文萍; 戈朝晖

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载异烟肼、利福平白蛋白纳米粒的制备及其体外释药研究

论著 | 更新时间：2025-01-15

- 载异烟肼、利福平白蛋白纳米粒的制备及其体外释药研究
- Preparation and in vitro release of albumin nanoparticles loaded with isoniazid and rifampicin
- 陆军军医大学学报 2013年35卷第21期页码：2336-2339
- 作者机构：
  
  宁夏医科大学：研究生院,药学院,总医院药剂科，总医院骨科
- 作者简介：
- 基金信息：
- DOI：
  中图分类号： R912;R944.9;R978.3
- 纸质出版日期：2013，
  
  网络出版日期：2013-11-15，
- 稿件说明：
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马荣, 陈振, 张惠勇, 等. 载异烟肼、利福平白蛋白纳米粒的制备及其体外释药研究[J]. 陆军军医大学学报, 2013,35(21):2336-2339.

MA RONG, CHEN ZHEN, ZHANG HUIYONG, et al. Preparation and in vitro release of albumin nanoparticles loaded with isoniazid and rifampicin. [J]. Journal of army medical university, 2013, 35(21): 2336-2339.
马荣, 陈振, 张惠勇, 等. 载异烟肼、利福平白蛋白纳米粒的制备及其体外释药研究[J]. 陆军军医大学学报, 2013,35(21):2336-2339. DOI：

MA RONG, CHEN ZHEN, ZHANG HUIYONG, et al. Preparation and in vitro release of albumin nanoparticles loaded with isoniazid and rifampicin. [J]. Journal of army medical university, 2013, 35(21): 2336-2339. DOI：

摘要

目的研究载异烟肼(isoniazid

INH)、利福平(rifampicin

RFP)牛血清白蛋白(bovine serum albumin

BSA)纳米粒的制备及体外释药特性。方法以白蛋白为载体聚乳酸为骨架，采用改良自乳化-溶剂扩散法制备纳米粒。采用透射电镜观察纳米粒形态；激光粒度衍射分析仪测定纳米粒粒径分布；高效液相色谱法测定纳米粒的载药量和包封率；动态膜透析法观察载药纳米粒的体外释药特性。结果制备的INH-RFP-BSA-NPs电镜下观察外观圆整，大小均匀、分散性好，粒径为（60.5±4.6）nm。异烟肼、利福平的载药量分别为19.8%、20.1%，包封率分别为87.8%、98%。INH至6 d时累计释放97.02%，RFP至5d时完全释放。结论以白蛋白和聚乳酸为载药基质，采用改良自乳化-溶剂扩散法可制备出粒径小、载药量和包封率较高的载异烟肼、利福平白蛋白纳米粒

体外释药显示载异烟肼、利福平白蛋白纳米粒具有良好的缓释作用。

Abstract

Objective To prepare of bovine serum albumin nanoparticles loaded with isoniazid and rifampicin (INH-RFP-BSA-NPs)and investigate its

in vitro

release characteristics. Methods INH-RFP-BSA-NPs were prepared by modified self-emulsion solvent diffusion method. Albumin and polylactic acid was used as a carrier as well as skeleton structure. Transmission electron microscopy was used to observe the morphology of INH-RFP-BSA-NPs. The nanoparticle’s size and distribution of INH-RFP-BSA-NPs were performed by submicron particle size analyzer. High performance liquid ch

romatography (HPLC) was used to measure the drug loadings and the coating rate of INH-RFP-BSA-NPs. Dynamic membrane dialysis was used to study the

in vitro

release characteristics of INH-RFP-BSA-NPs. Results The INH-RFP-BSA-NPs were smooth

sphere-like

relatively uniform in size and good dispersion. The submicron particle size analyzer showed the diameter was 60.5±4.6 nm. The drug loading and entrapment efficiency of isoniazid were 19.8% and 87.8% respectively

and those of rifampicin were 20.1% and 98% respectively

INH cumulative release of 97.02% to 6 d

RFP to 5 d fully released. Conclusion INH-RFP-BSA-NPs can be prepared by drug-loading with albumin and polylactic acid

matrix modified self-emulsion solvent diffusion

with higher encapsulation efficiency

higher drug loading

and smaller particle size.

In vitro

drug release shows INH-RFP-BSA-NPs has good slow release effect.

关键词

白蛋白异烟肼利福平纳米粒体外释药

Keywords

albuminisoniazidrifampicinnanoparticlesin vitro drug release

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