Chen Hongxiang. Screening of potential molecular markers in cervical cancer cells by ultra-high-speed cell selection platform combined with cDNA microarray technology[J]. Journal of chongqing medical university, 2023, 48(1): 54-60.
Chen Hongxiang. Screening of potential molecular markers in cervical cancer cells by ultra-high-speed cell selection platform combined with cDNA microarray technology[J]. Journal of chongqing medical university, 2023, 48(1): 54-60.DOI:
Objective: To screen potential molecular markers of cervical cancer cells by ultra-high speed cell sorting platform combined with cDNA microarray technology. Methods: In this paper
the MoFlo XDP-type ultra-high-speed cell selection platform was used to purify cervical cancer cells expressing CD38 and not expressing CD38 on the cell membrane surface. RNAlater technology was used to obtain the RNA required for cDNA microarray experiments
and then by gene chip analysis was made. Results: The MoFlo XDP-type ultra-high-speed cell selection platform can be used to obtain cervical cancer cells with a CD38-positive expression of 99.0% purity. Conclusion: Through the analysis of cDNA microarray
potential molecular markers of cervical cancer cells such as RORA
PLIN4
AUTS2
and IFITM1 have been found
which provides a new technical method for the research of cervical cancer.