肉桂醛促进高糖条件下皮肤成纤维细胞迁移及机制研究

李文杰; 蔡雷琴; 隆敏; 郑宏庭

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肉桂醛促进高糖条件下皮肤成纤维细胞迁移及机制研究

基础医学 | 更新时间：2026-01-09

- 肉桂醛促进高糖条件下皮肤成纤维细胞迁移及机制研究
- Cinnamic aldehyde improves fibroblasts migration under hyperglycaemic condition and its underlying mechanism
- 陆军军医大学学报 2017年39卷第5期页码：423-429
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  中图分类号： R285.5;R322.99;R587.1
- 纸质出版：2017
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李文杰,蔡雷琴,隆敏,郑宏庭.肉桂醛促进高糖条件下皮肤成纤维细胞迁移及机制研究[J].陆军军医大学学报(原第三军医大学学报),2017,39(05 ):423-429.　Li Wenjie,Cai Leiqin,Long Min,Zheng Hongting.Cinnamic aldehyde improves fibroblasts migration under hyperglycaemic condition and its underlying mechanism[J].J Amry Med Univ (J Third Mil Med Univ),2017,39(05 ):423-429.

李文杰, 蔡雷琴, 隆敏, et al. Cinnamic aldehyde improves fibroblasts migration under hyperglycaemic condition and its underlying mechanism[J]. Journal of Army Medical University, 2017, 39(5): 423-429.
李文杰,蔡雷琴,隆敏,郑宏庭.肉桂醛促进高糖条件下皮肤成纤维细胞迁移及机制研究[J].陆军军医大学学报(原第三军医大学学报),2017,39(05 ):423-429.　Li Wenjie,Cai Leiqin,Long Min,Zheng Hongting.Cinnamic aldehyde improves fibroblasts migration under hyperglycaemic condition and its underlying mechanism[J].J Amry Med Univ (J Third Mil Med Univ),2017,39(05 ):423-429. DOI：

李文杰, 蔡雷琴, 隆敏, et al. Cinnamic aldehyde improves fibroblasts migration under hyperglycaemic condition and its underlying mechanism[J]. Journal of Army Medical University, 2017, 39(5): 423-429. DOI：

摘要

目的探讨肉桂醛（cinnamic aldehyde，CA），核因子E2相关因子（nuclear factor erythroid2 related factor，NRF2）通路激动剂对高糖条件下成纤维细胞迁移的影响及可能机制。方法体外培养HS27细胞，分为正常对照组（NG，5.5 mmol/L）、高糖组（HG，30 mmol/L）、高糖（30 mmol/L）+肉桂醛（20 μmol/L）组、高糖（30 mmol/L）+Con siRNA组、高糖（30 mmol/L）+NRF2 siRNA组。迁移实验观察CA对高糖条件下HS27迁移的影响。采用western blot分析CA干预后NRF2及下游HO-1，增殖和凋亡相关蛋白P21和P53的表达情况。2′，7′二氯荧光素二醋酸（DCF）法检测CA对高糖条件下细胞内活性氧簇（reactive oxygen species，ROS）水平的影响。采用NRF2siRNA抑制NRF2通路，观察HS27细胞迁移和氧化应激的变化。结果与正常对照组相比，HS27在高糖情况下，迁移能力明显降低(P0.05），CA干预后，高糖对HS27迁移能力的抑制作用得到改善（P0.05）。进一步研究发现，CA上调NRF2和HO-1，减轻高糖导致的ROS增加（[（4 360 935±1 445 723）vs（1 982 673±208 109），P0.05]）。然而，高糖条件下敲除NRF2后，细胞内ROS产生进一步增多，并加剧对成纤维细胞迁移能力的抑制[（0.046±0.018）vs（0.318±0.032），P0.05]。结论 CA促进高糖情况下成纤维细胞迁移、缓解氧化应激损伤，并有可能部分依赖于NRF2通路激活。

Abstract

Objective To determine the effect of cinnamic aldehyde (CA)

a nuclear factor erythroid 2-related factor (NRF2) pathway activator

on the migration of fibroblasts (HS27 cells) and investigate the potential mechanism. Methods HS27 cells were divided into 5 groups

and were treated by normal glucose (NG

5.5 mmol/L)

high glucose (HG

30 mmol/L)

high glucose and CA (20 μmol/L)

high glucose and Con siRNA

high glucose and NRF2 siRNA

respectively. Polydimethylsiloxane (PDMS) assay was used to determine the effect of CA on cell mobility under high glucose condition. Western blotting was employed to examine the effect of CA on the expression of NRF2 and its downstream molecule HO-1

and proliferation and apoptosis related P21 and P53. Intracellular reactive oxide species (ROS) were measured in HS27 cells treated with CA using 2′

7′dichlorofluorescein diacetate (DCF) assay. NRF2 siRNA were transfected into HS27 cells to further validate the role of NRF2 pathway in the regulation of cell migration and oxidative stress. Results Compared with the HS27 cells under normal glucose condition

the migration of the cells cultured in high-glucose medium were significantly inhibited (P0.05). After treatment with CA

the cell mobility was significantly improved (P0.05) and the expression levels of NRF2 and HO-1 were notably increased. Meanwhile

CA treatment significantly ameliorated hyperglycemia-induced ROS production in HS27 cells (4 360 935±1 445 723 vs 1 982 673±208 109

P0.05). However

knocking down of NRF2 far more enhanced ROS levels

and aggravated inhibitory effects of CA on migration of HS27 cells under high glucose condition (0.046±0.018 vs 0.318±0.032

P0.05). Conclusion CA treatment promotes the migration of fibroblasts and alleviates the oxidative stress damage from high glucose culture

which might be partially due to the activation of NRF2 pathway.

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