锌指蛋白A20及其相关炎症因子在椎间盘髓核细胞退变前后的表达变化

刘浠; 易威威; 温亚枫; 叶禾; 谭富强; 刘渤

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锌指蛋白A20及其相关炎症因子在椎间盘髓核细胞退变前后的表达变化

临床医学 | 更新时间：2026-01-09

- 锌指蛋白A20及其相关炎症因子在椎间盘髓核细胞退变前后的表达变化
- Expression of zinc finger protein A20 and related inflammatory factors in nucleus pulposus cells in degenerative intervertebral disc
- 陆军军医大学学报 2016年38卷第18期页码：2077-2081
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  中图分类号： R341;R363.22;R681.53
- 纸质出版：2016
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刘浠,易威威,温亚枫,叶禾,谭富强,刘渤.锌指蛋白A20及其相关炎症因子在椎间盘髓核细胞退变前后的表达变化[J].陆军军医大学学报(原第三军医大学学报),2016,38(18):2077-2081.

　Liu Xi,Yi Weiwei,Wen Yafeng,Ye He,Tan Fuqiang,Liu Bo.Expression of zinc finger protein A20 and related inflammatory factors in nucleus pulposus cells in degenerative intervertebral disc[J].J Amry Med Univ (J Third Mil Med Univ),2016,38(18):2077-2081.
刘浠,易威威,温亚枫,叶禾,谭富强,刘渤.锌指蛋白A20及其相关炎症因子在椎间盘髓核细胞退变前后的表达变化[J].陆军军医大学学报(原第三军医大学学报),2016,38(18):2077-2081. DOI：

　Liu Xi,Yi Weiwei,Wen Yafeng,Ye He,Tan Fuqiang,Liu Bo.Expression of zinc finger protein A20 and related inflammatory factors in nucleus pulposus cells in degenerative intervertebral disc[J].J Amry Med Univ (J Third Mil Med Univ),2016,38(18):2077-2081. DOI：

摘要

目的检测椎间盘髓核细胞退变前后锌指蛋白A20及其相关炎症因子的表达变化。方法获取我院骨科患者术中切除的椎间盘组织，分为正常组和退变组，采用阿辛蓝染色观察人椎间盘髓核细胞的退变情况，免疫组化观察人椎间盘髓核细胞A20、TNF-α、IL-1β及NF-κB p65蛋白的表达。体外培养胚胎小鼠髓核细胞，分为对照组(只加入等量的PBS)、内毒素(LPS)刺激组和NF-κB抑制组，分别以Real-time PCR检测LPS刺激细胞和加入NF-κB抑制剂后，A20、TNF-α、IL-1β、Ⅰ型血小板结合蛋白基序的解聚蛋白样金属蛋白酶-4（ADAMST-4）、Ⅰ型血小板结合蛋白基序的解聚蛋白样金属蛋白酶-5（ADAMST-5）、基质金属蛋白酶-13（MMP-13）的表达变化；Western blot检测A20、TNF-α、IL-1β、ADAMTS-4、ADAMTS-5、MMP-13、p65、磷酸化p65（p-p65) 的表达变化。结果退变组髓核细胞数量显著少于正常组，并有明显的聚集现象；退变组髓核细胞中A20、TNF-α、IL-1β、p65蛋白表达明显高于正常组；LPS刺激胚胎小鼠髓核细胞后，LPS刺激组A20、TNF-α、IL-1β、ADAMST-4、ADAMST-5、MMP-13 mRNA与蛋白的表达均高于NF-κB抑制组与对照组（P0.05），A20、TNF-α、IL-1β、ADAMTS-4、ADAMTS-5、MMP-13、p65、p-p65蛋白含量也较NF-κB抑制组与对照组显著增高，差异有统计学意义（P0.05）。结论髓核细胞炎症反应与椎间盘退变的发生、发展密切相关， A20可能通过NF-κB信号通路对抑制髓核细胞退变时炎症反应起到关键作用。

Abstract

Objective To study the expression of zinc finger protein A20 and related inflammatory factors in nucleus pulposus cells in degenerative intervertebral disc. Methods Human intervertebral disc tissues provided by department of orthopedics in our hospital were categorized into normal and degenerative groups. The degenerative degree of nucleus pulposus cells was evaluated by Alcian blue staining. The expression of A20

TNF-α

IL-1β and p65 in nucleus pulposus cells was detected by immunohistochemical (IHC) staining. In vitro embryonic mouse nucleus pulposus cells were cultured. Based on the treatment

the cells were divided into control group

LPS stimulation group and NF-κB inhibition group. Real-time PCR (RT-PCR) was performed to measure the mRNA expression of A20

TNF-α

IL-1β

ADAMST-4

ADAMST-5

and MMP-13. Western blotting was used to detect the protein expression of A20

TNF-α

IL-1β

ADAMTS-4

ADAMTS-5

MMP-13

p65

and p-p65. Results In human degenerative intervertebral disc

the number of nucleus pulposus cells was obviously decreased in favor of aggregation as compared to the normal group. Also

the expression of A20 was significantly higher than the normal group detected by IHC staining. In embryonic mouse nucleus pulposus cells

the mRNA expression of A20

TNF-α

IL-1β

ADAMST-4

ADAMST-5 and MMP-13 was significantly higher in LPS stimulation group than that in NF-kB inhibition group and control group (P0.05). Likely

LPS stimulation increased the protein levels of A20

TNF-α

IL-1β

ADAMST-4

ADAMST-5

MMP-13

p65 and p-p65 compared to NF-kB inhibition group and control group

which was of statistic significance (P0.05). Conclusion The inflammatory response of nucleus pulposus cells is closely related to the occurrence and development of intervertebral disc degeneration

which A20 may play an important role in inhibiting this inflammatory reaction through NF-kB signaling pathway.

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