Objective      To determine the effect of dapper antagonist of catenin-1 (DACT1) on malignant invasion in type Ⅰ ovarian cancer and investigate the plausible mechanism. Methods     The expression of DACT1 in type Ⅰ ovarian cancer tissue samples was detected by immunohistochemical assay. Human mucous ovarian cancer 3AO cells which expressed DACT1 at low endogenous level

were transfected by the lentivirus vectors carrying full-length DACT1. Wound healing

Transwell chamber assay and matrigel invasion assay were performed to measure cell motility. F-actin was detected by immunofluorescence assay for the effect of DACT1 on cytoskeletal remodeling. ResultsExpression of DACT1 in type Ⅰ ovarian cancer was significantly lower in ovarian cancer tissues than in normal ovarian tissues (P0.01). Up-regulation of DACT1 significantly inhibited the migration and invasion capacities (P0.01)

intraperitoneal tumorigenesis dissemination

and specialized membrane structure in the cytoskeleton the 3AO cells. Conclusion      DACT1 inhibits invasion and motility in type Ⅰ ovarian cancer

and its low expression may be associated with the pathogenesis of type Ⅰ epithelial ovarian cancer.