Objective      To analyze the correlation between serum Klotho protein level and inflammatory factors in the patients with chronic kidney disease (CKD) for assessment of Klotho protein associated microinflammatory state. Methods     ELISA was used to measure the levels of serum Klotho protein

C-reactive protein (CRP)

interleukin-6 (IL-6) and tumor necrosis factor α (TNF-α) in 314 patients with stages 1~5 CKD. Serum albumin (ALB)

blood phosphorus (P)

serum creatinine (Scr)

uric acid (UA) and cystatin (Cys-c) were examined by bioanalysis. Patients’ age

sex

height

weight and other indicators were recorded. Estimated glomerular filtration rate (eGFR) was calculated according to CKD-EPI equation. The serum levels of inflammatory factors in different stages of CKD were compared by single factor analysis of variance (ANOVA).The correlation of inflammatory factors with ALB

eGFR

UA

Klotho and P was analyzed by Spearman’s rank correlation. The parameters influencing the inflammatory factors in the CKD patients were analyzed by multiple linear regression analysis. Results      ① ANOVA analysis showed that in the patients with stages 1~5 CKD

serum TNF-α was increased gradually (P0.01)

CRP and IL.6 had no significant difference; Klotho protein level was reduced along with the CKD stages (P0.01). ② Correlation analysis indicated that serum TNF-α levels in patients with CKD was negatively correlated with eGFR (r=-0.550，P0.01)and Klotho (r=-0.753，P0.01)

respectively

whereas Klotho protein and eGFR were positively correlated (r=0.643，P0.01) . ③ Multiple linear regression analysis demonstrated a strong relationship of serum TNF-α with both Klotho and eGFR in CKD patients. Conclusion     Our data showed significantly decreased level of serum Klotho and increased level of inflammatory cytokine TNF.α in CKD patients with decreasing eGFR. The strong correlation between Klotho protein and inflammatory factors suggests that the decrease of Klotho protein is closely related to the occurrence and development of micro-inflammatory state in patients with CKD.