miR200c过表达对寡灶型转移细胞株体外特性的影响

陈俊良; 孙志卫; 王健宇; 邢若曦

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miR200c过表达对寡灶型转移细胞株体外特性的影响

基础医学 | 更新时间：2026-01-09

- miR200c过表达对寡灶型转移细胞株体外特性的影响
- Effect of miR200c over-expression on biological features of oligometastatic cells in vitro
- 陆军军医大学学报 2016年38卷第23期页码：2486-2491
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  中图分类号： R394.3;R73354;R730.23
- 纸质出版：2016
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陈俊良,孙志卫,王健宇,邢若曦.miR200c过表达对寡灶型转移细胞株体外特性的影响[J].陆军军医大学学报(原第三军医大学学报),2016,38(23):2486-2491.

　Chen Junliang,Sun Zhiwei,Wang Jianyu,Xing Ruoxi.Effect of miR200c over-expression on biological features of oligometastatic cells in vitro[J].J Amry Med Univ (J Third Mil Med Univ),2016,38(23):2486-2491.
陈俊良,孙志卫,王健宇,邢若曦.miR200c过表达对寡灶型转移细胞株体外特性的影响[J].陆军军医大学学报(原第三军医大学学报),2016,38(23):2486-2491. DOI：

　Chen Junliang,Sun Zhiwei,Wang Jianyu,Xing Ruoxi.Effect of miR200c over-expression on biological features of oligometastatic cells in vitro[J].J Amry Med Univ (J Third Mil Med Univ),2016,38(23):2486-2491. DOI：

摘要

目的探索miR200c稳定过表达对寡灶型转移肿瘤细胞株体外癌症生物学特性的影响。方法通过慢病毒感染建立稳定上调miR200c表达的寡灶型转移细胞株MDA-435-OL-miR200c（实验组），并同时建立阴性对照组（感染空质粒LV3NC）和阳性对照组（多灶型细胞株MDA-435-POL），通过CCK-8增殖曲线测定、琼脂平板克隆实验、Transwell小室迁移和侵袭实验来阐明上调miR200c对寡灶型转移细胞体外特性的影响以及上调目的基因后寡灶型与多灶型转移细胞株体外癌症生物学特性的不同和联系。结果在稳定寡灶转移细胞株中过表达miR200c对细胞体外克隆形成率为（0.69±0.02），迁移细胞数为（21.20±2.35），侵袭细胞数为（56.80±5.22），均较阴性对照组显著增加（P0.05），与阳性对照组体外特性基本一致。但miR200c过表达对增殖能力没有显著的影响。结论寡灶型细胞株稳定上调miR200c后体外克隆形成能力、迁移能力和侵袭能力均有所提高，且呈现出多灶型细胞株的特性。

Abstract

Objective To determine the effect of stable miR200c over-expression on the cancer biological features of the oligometastatic cells in vitro. MethodsAn oligometastatic cell line over-expressing miR200c was established by lentivirus infection (MDA-435-OL-miR200c). At the same time

a negative control cell line was generated by transfection with empty plasmid LV3NC. MDA-435-POL line

with confirmed polymetastatic phenotype in vivo served as a positive control for this study. The biological effect of miR200c over-expression was evaluated by CCK-8 assay for cell proliferation

agar colony formation assay for clonogeneicity

transwell migration assay and matrigel invasion assay for the invasiveness. Results Over-expression of miR200c resulted in the increases in the clonogenicity (cloning efficiency: 0.69±0.02)

cell migration (migrated cells number: 21.20±2.35) and invasion ability (invasive cells number: 56.80±5.22)

in the oligometastatic MDA-435-OL cells

and all the parameters were significantly higher than those of negative control (P0.05) and were similar to those of positive control. However

the miR200c over-expression had no effect on cell proliferation. Conclusion Stable over-expression of miR200c results in increases in clonogenicity

cell migration and invasion ability in the oligometastatic MDA-435-OL cells

which are resemble to those of MDA-435-POL polymetastatic cells.

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