乌鲁木齐地区金黄色葡萄球菌分离株的分子分型与耐药性分析

杨延成; 程航; 胡珍; 袁文常; 尚伟龙; 饶青; 胡启文; 杨杰; 刘正祥; 袁吉振; 张骁鹏; 彭华刚; 刘慧; 朱军民; 伏建峰; 饶贤才

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乌鲁木齐地区金黄色葡萄球菌分离株的分子分型与耐药性分析

公共卫生与预防医学 | 更新时间：2026-01-09

- 乌鲁木齐地区金黄色葡萄球菌分离株的分子分型与耐药性分析
- Molecular typing and antimicrobial resistances of Staphylococcus aureus isolated from a hospital in Urumqi, China
- 陆军军医大学学报 2016年38卷第9期页码：1035-1042
- 作者机构：
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  中图分类号： R181.34;R372;R378.11
- 纸质出版：2016
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杨延成,程航,胡珍,袁文常,尚伟龙,饶青,胡启文,杨杰,刘正祥,袁吉振,张骁鹏,彭华刚,刘慧,朱军民,伏建峰,饶贤才.乌鲁木齐地区金黄色葡萄球菌分离株的分子分型与耐药性分析[J].陆军军医大学学报(原第三军医大学学报),2016,38(09):1035-1042.

　Yang Yancheng,Cheng Hang,Hu Zhen,Yuan Wenchang,Shang Weilong,Rao Qing,Hu Qiwen,Yang Jie,Liu Zhengxiang,Yuan Jizhen,Zhang Xiaopeng,Peng Huagang,Liu Hui,Zhu Junmin,Fu Jianfeng,Rao Xiancai.Molecular typing and antimicrobial resistances of Staphylococcus aureus isolated from a hospital in Urumqi, China[J].J Amry Med Univ (J Third Mil Med Univ),2016,38(09):1035-1042.
杨延成,程航,胡珍,袁文常,尚伟龙,饶青,胡启文,杨杰,刘正祥,袁吉振,张骁鹏,彭华刚,刘慧,朱军民,伏建峰,饶贤才.乌鲁木齐地区金黄色葡萄球菌分离株的分子分型与耐药性分析[J].陆军军医大学学报(原第三军医大学学报),2016,38(09):1035-1042. DOI：

　Yang Yancheng,Cheng Hang,Hu Zhen,Yuan Wenchang,Shang Weilong,Rao Qing,Hu Qiwen,Yang Jie,Liu Zhengxiang,Yuan Jizhen,Zhang Xiaopeng,Peng Huagang,Liu Hui,Zhu Junmin,Fu Jianfeng,Rao Xiancai.Molecular typing and antimicrobial resistances of Staphylococcus aureus isolated from a hospital in Urumqi, China[J].J Amry Med Univ (J Third Mil Med Univ),2016,38(09):1035-1042. DOI：

摘要

目的了解乌鲁木齐地区金黄色葡萄球菌临床分离株的分子特征及其对临床常用抗生素的耐药情况。方法收集2010年6月至2012年12月期间乌鲁木齐地区一所大型综合医院临床分离的86株金黄色葡萄球菌，在鉴定区分耐甲氧西林金黄色葡萄球菌(methicillin-resistant Staphylococcus aureus，MRSA)和甲氧西林敏感金黄色葡萄球菌(methicillin-susceptible Staphylococcus aureus，MSSA)的基础上，应用SCCmec分型、spa分型、多位点序列分型(multilocus sequence typing，MLST)、脉冲场凝胶电泳分型(pulsed-field gel electrophoresis，PFGE)、agr分型等葡萄球菌分子分型方法进行分析，检测pvl毒力基因的携带率，检测菌株对苯唑西林、红霉素、四环素等13种临床常用抗生素的耐药性，分析菌株的耐药情况及耐药基因。结果 86株金葡菌中31株为MRSA，55株为MSSA。31株MRSA分为8种spa型和7种ST型，优势流行克隆为ST239-MRSA-Ⅲ-t030 (71%， 22/31)。55株MSSA分为23种spa型、16种ST型和8个PFGE聚类组，agrⅠ型占56.4% (31/55)。MSSA菌株的pvl毒力基因检出率为49.1%(27/55)，MRSA 为38.7%(12/31)。药敏结果显示31株MRSA均为多重耐药(multidrug-resistant，MDR)菌株，55株MSSA中有31株为MDR菌株。结论乌鲁木齐地区流行的MRSA以ST239-MRSA-Ⅲ-t030为主，而MSSA表现出较高遗传多样性，发现有t11413，ST121等新的型别流行。

Abstract

Objective To determine the molecular types and resistance of Staphylococcus aureus (S. aureus) strains to commonly used antibiotics isolated in Urumqi

China. Methods A total of 86 S. aureus isolates were collected from a large-scale general hospital in Urumqi during June 2010 and December 2012. The methicillin-resistant S. aureus (MRSA) and methicillin-susceptible S. aureus (MSSA) were determined. The molecular typing

including staphylococcal cassette chromosome mec (SCCmec) typing

staphylococcal protein A (spa) gene typing

multilocus sequence typing (MLST)

pulsed-field gel electrophoresis (PFGE)

and accessory gene regulator (agr) typing were applied for the strains. The carriage of Panton-Valentine Leukocidin (pvl) genes and drug susceptibilities of S. aureus strains against 13 kinds of antibiotics were determined. Results In the 86 S. aureus isolates

31 were MRSA (36.0%) and 55 were MSSA (64.0%). The MRSA strains were divided into 8 spa types and 7 ST types

ST239-MRSA-Ⅲ-t030 was the most prevalent clone (71%

22/31). Among 55 MSSA strains

23 spa types

16 ST types and 8 PFGE groups were found. Most MSSA strains were agrⅠ types (56.4%

31/55). The frequency of pvl carriage was 49.1% (27/55) in MSSA strains

and was 38.7% (12/31) in MRSA strains. All MRSA strains were multidrug-resistant (MDR)

whereas 31 out of the 55 MSSA strains (56.4%) belonged to MDR. Conclusion ST239-MRSA-Ⅲ-t030 is still the most predominant MRSA in Urumqi. MSSA isolates show wide genetic diversity. New types t11413 and ST121 are also dominant.

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