Objective      To explore the effect of lipopolysaccharide (LPS) exposure during pregnancy on the expression and function o

f renal angiotensin Ⅱ type 1 receptor (AT

1

R) in the adult rat offsprings

and provide new evidence for prevention and treatment of hypertension.     Methods      SD female rats (20 weeks old) with body weight of 250~280 g were mated with SD male rats (20 weeks old) with body weight of 250~280 g

and the female rats with vaginal plug of white crystalline particles were identified as the pregnant rats. Twelve pregnant SD rats were randomly divided into LPS group (n=6) and control group (n=6). On days 8

10

and 12 after gestation

the LPS group was treated by intraperitoneal injection of 0.79 mg/kg LPS

while the control group was injected with the equal volume of normal saline. Blood pressure of the offsprings was measured by noninvasive tail pressure instrument

and the urine of the offsprings was collected by metabolic cage. The renal AT

1

R function of the offsprings was determined by suprarenal artery administration of AT

1

R antagonist candesartan. The renal AT

1

R mRNA level was measured by qRTPCR

while the protein expression was detected by Western blotting.         Results          With the increasing age

the blood pressure of the offsprings was significantly higher in the LPS group than in the control group. 24h urine results showed that the urine volume and urinary sodium excretion rate of the offsprings from the LPS group were lower than those from the control group (24.6±1.8 vs 19.5±1.5 mL

750.7±48.2 vs568.4±52.5  mol/d

P0.05). The candesartaninduced natriuretic and diuretic effects in the offsprings from the LPS group were stronger than those in the offsprings from the control group (6.5±0.6 vs11.7±1.3 mL

856.8±61.6 vs1 282.4±89.9  mol/min

P0.05). The expression of AT1R at mRNA and protein levels in the offsprings from the LPS group were higher than those from the control group (1.31±0.07 vs 0.56±0.06

1.41±0.03 vs 0.58±0.12

P0.05).   Conclusion     LPS exposure during pregnancy can induce hypertension in the adult rat offsprings

and it may be relat

ed with high expression and function of AT

1

R in the kidney

which leads to retention of water and sodium.