Objective       To determine the effect of deficiency of xeroderma pigmentosum group C (XPC) on the autophagy in bladder cancer cells.       Methods       By using shRNA

a stable XPC deficient cell model was established based on human bladder cancer T24 cells. The protein level of LC3 Ⅱ was detected by Western blotting to monitor the autophagy. GFP-LC3 puncta were visualized by using fluorescence microscopy after transiently transfected with a GFP-LC3 expression vector. Cell viability was detected by CCK-8 assay. The expression of the proteins involved in apoptosis and autophagy signaling was detected by Western blot assay.       Results      A stable XPC deficient model of T24 cells was successfully established. Knocking down of XPC resulted in decreases of autophagosome formation under both normal [(1.53±0.81)% vs (3.30±0.70)%

P0.05] and cisplatin-induced DNA damage condition [(2.19±0.37)% vs (3.20±0.29)%

P0.05]. Cisplatin promoted cell autophagy in XPC deficient T24 cells. Autophagy played a non-protective role in XPC deficient cells when dealing with cisplatin induced DNA damage.       Conclusion      XPC is involved in the autophagy in T24 cells.