Objective      To determine the effect of specific neurotransmitters on hepatocyte recellularization in prepared decalularized liver bioscaffold.       Methods      Decalularized liver bioscaffold was prepared from the liver of adult C57 mouse with whole organ decalularization. The obtained bioscaffolds were detected by HE and Massion staining

and the quantification of residual DNA was detected. Real-time PCR and immunofluorescence assay were used to detect the expression of adrenoceptors and subtypes in L02 cell line (seed cells). The proliferation of the seed cells cultured in the plate or the bioscaffold was measured in presence of isoproterenol. The secretion of albumin and urea was detected after isoproterenol treatment.       Results      Decalularized liver bioscaffold was successfully prepared. Adrenoceptors were expressed in the L02 cells

with its subtype adrenoceptor beta2 expressed relatively highest. Isoproterenol

as an adrenoceptor beta agonist

promoted the proliferation of the hepatocytes both in plane and bioscaffold

and the secretion of albumin and urea in the recellularized liver.       Conclusion      Isoproterenol promotes the proliferation of hepatocytes in decalularized liver bioscaffold and the function of recellularized liver.