Objective      To determine whether resveratrol (RSV) attenuates Aβ

25-35

induced neurotoxicity through SIRT1/autophagy signaling pathway.       Methods      PC12 cells were treated with different concentrations (1

5

10 and 20 μmol/L) of RSV for 2 h in the presence or absence of 5 mmol/L 3-methyladenine (3-MA

specific inhibitor of autophagy ) or 2 μmol/L EX527 (selective inhi

bitor of SIRT1)

following by treatment of 30 μmol/L amyloid β-protein fragment 25-35 (Aβ

25-35

) for 24 h. CCK-8 assay was used to detect the cell viability

and electron microscope was applied to detect autophagosome. The expressions of P62

LC3 and SIRT1 were detected by Western blotting.       Results      Aβ

25-35

(30 μmol/L) remarkably decreased the viability of PC12 cells

which was 45.14% of the control group P0.05). Pretreatment with RSV significantly improved Aβ

25-35

induced decrease of cell viability in a dose-dependent manner (P0.05). Simultaneously

RSV promoted the formation of autophagosome

up-regulated the expressions of SIRT1 and LC3-Ⅱ and down-regulated the expression of P62 in a dose-dependent manner. However 3-MA and EX527 abolished the above effects of RSV.       Conclusion      RSV attenuates Aβ

25-35

induced neurotoxicity through activation of SIRT1/autophagy signaling pathway.