Objective      To investigate the effect of miR-378 on apoptosis and proliferation in myelodysplastic syndrome SKM-1 cells.       Methods      SKM-1 cells were divided into 3 groups: SKM-1 cells transfected with lentiviral vector over-expressing miR-378 (miR-378 group)

SKM-1 cells transfected with empty lentiviral vector (negative control group) and normal SKM-1 (blank control group). CCK-8 assay and Annexin V/7-AAD staining were conducted to detect the effect of miR-378 on the proliferation and apoptosis of SKM-1 cells. Then

Western blotting was performed to detect the expression of cleaved-capase-3 protein. Bioinformatics analysis was conducted to predict the potential target gene of miR-378

and quantitative reverse transcriptase-polymerase chain reaction and Western blotting were further confirm the target gene.       Results      After transfection with lentiviral vector over-expressing miR-378

the growth of SKM-1 cells in the miR-378 group was inhibited obviously. The apoptotic rate [(12.90 ± 3.72)%] in the miR-378 group was significantly higher than [(3.21 ± 1.91)%] in the negative control group and [(2.78 ± 1.04)%] in the blank control group (P0.01). The percentage of SKM-1 cells in G0/G1 phase was increased and the percentage in S phase was decreased. Western blotting showed that overexpression of miR-378 resulted in up-regulation of cleaved-caspase-3. Moreover

BCL2L2 gene was predicted as potential target gene of miR-378

and overexpression of miR-378 reduced the expression of BCL2L2 protein.       Conclusion      miR-378 can inhibit proliferation of SKM-1 cells by inducing apoptosis and cell cycle arrest

and it also down-regulates the expression of BCL2L2 protein.