Objective      To investigate the changes of extracellular metabolites of drug-resistant oral squamous cell carcinoma Tca8113 cells

and look for different metabolites.       Methods      Samples of extracellular metabolites were prepared. The spectra of extracellular metabolites of Tca8113 cells and drug-resistant Tca8113/CBP cells were obtained by using

1

H NMR. Different variables with greater contribution to cell discrimination were found using partial least squares-discriminant analysis (PLS-DA)

and the metabolites which would meet both VIP1 and univariate statistic P0.05 were identified as the ultimate different metabolites.       Results     

1

H NMR-based metabolomics method cou

ld distinguish Tca8113 cells and Tca8113/CBP cells. The ultimate different metabolites contained acetate

taurine

serine

glucose and leucine

which were involved in metabolism of protein

carbohydrate metabolism and tricarboxylic acid cycle.       Conclusion      Application of metabolomics successfully find different metabolites of drug-resistant Tca8113 cells.