Objective      To validate a novel nonsense somatic mutation in MSH2 of a patient with primary hepatocellular carcinoma using droplet digital PCR (ddPCR).       Methods      ddPCR was used to validate a novel nonsense mutation in MSH2 identified by whole exome sequencing. Qualitative and quantitative analyses were performed in the liver cancer tissues and adjacent non-cancerous tissues.       Results      ddPCR validated that the nonsense mutation (rs63751009

g. chr2:47630358 CT

c.28 CT

p.Q10*) in MSH2 occurred in the liver cancer tissues rather than the adjacent non-cancerous tissues. The allelic fraction of the nonsense mutation obtained from ddPCR was 0.4

which was similar with that estimated from the whole-exome sequencing.       Conclusion       ddPCR is a new

rapid and accurate method for mutation analysis with high sensitivity and accuracy.