Objective       To compare the physicochemical properties and biological compatibility of α-calcium sulfate hemihydrates (α-CSH)/β-tricalcium phosphate (β-TCP) composite artificial bone materials prepared by different preparation processes and calcium sulfate material Osteoset purchased from WRIGHT company

in order to provide theoretical basis and experimental basis for the development and improvement of new artificial bone materials.        Methods       X-ray diffraction analysis

morphology observation

in vitro cytotoxicity test

hemolysis test

and pyrogen reaction were adopted to detect physicochemical properties of α-CSH/β-TCP composite artificial bone materials (divided into A group and B group according to different preparation processes) and Osteoset group (C group). Artificial bone material muscle embedding and histological examination were performed to test the biological compatibility of the 3 kinds of materials.        Results       X-ray diffraction analysis found that the 3 kinds of materials had own characteristic diffraction peaks and no new peaks. Microcellular structure observation suggested that each group showed more interconnected parallel pores

uniform pore distribution

good pore penetrating structure

and relatively uniform and consistent crystal structure and morphology. MTT assay found that: the RGR value of group A

B

C were greater than 75% in different time point

and the Toxicity grading for level Ⅰ

Toxicity level 0 for negative control group

and level Ⅳ for positive control group. Hemolytic experiment shows: The average rate of hemolysis of each group was 0.95%

2.22%

1.05% respectively. The body temperature of each group rise 0.2 ℃

0.1~0.2 ℃

0.1~0.2 ℃ in different time point. And the sum rise total was 0.4 ℃

0.3 ℃

0.3 ℃ respectively. Under optical microscope the growth structure of muscle fiber turns normal

without any inflammatory cell infiltration and rejection reaction.The cell growth and proliferation were not affected

and the hemolysis

pyrogen reaction and inflammatory reaction were not found.        Conclusion       The 2 kinds of homemade composite artificial bone materials show good biocompatibility

and have potential clinical application prospect.