Objective      To determine the effect of formyl peptide receptor 1 (FPR1) on the migration of BV2 cells and investigate the underlying mechanism.       Methods       The expression of FPR1 receptor in BV-2 cells was detected by immunofluorescence staining. Transwell assay was used to detect the effect of FPR1 receptor specific agonist

formylmethionyl-leucyl-phenylalanine (fMLP)

at different concentrations (1

10

50

100 and 500 nmol/L) on the alterations of cell migration

while the scratch test and Transwell assay were used to verify the role of fMLP in the promotion of cell migration whether can be inhibited by FPR1 specific antagonist

Boc-MLF

or not. The protein expression of FPR1 was detected by Western blotting in the BV-2 cells stimulated with different concentrations of fMLP. After stimulated with 100 nmol/L fMLP and 5 μmol/L Boc-MLF

the expression of phospho-extracellular regulate kinase (p-ERK) in the cells was detected by Western blotting.       Results      Immunofluorescence staining indicated that FPR1 receptor was expressed in BV-2 cells

mainly located in the cell membrane. With the increase of fMLP doses

Transwell assay showed that the migration of BV-2 cells was enhanced significantly (P0.05) in a dose-dependent fashion. The 100 and 500 nmol/L fMLP showed most obvious effect

while the optimal fMLP concentration to promote BV-2 cell migration was 100 nmol/L. The scratch test and Transwell assay verified that those above effects could be inhibited by 5 μmol/L Boc-MLF (P0.05). The specific FPR1 agonist

fMLP up-regulated the FPR1 receptor expression in BV-2 cells (P0.05)

activated ERK signaling pathway

and increased p-ERK protein expression significantly (P0.05). The FPR1 specific antagonist Boc-MLF inhibited the above changes significantly (P0.05).       Conclusion      FPR1 receptor is expressed in the BV-2 cells

and FPR1 plays an important role in the promotion of BV-2 cells migration.