6-姜烯酚通过下调TLR4/NF-κB通路抑制血管平滑肌细胞增殖

陈磊; 胡子成; 周毅; 张明杰; 王旭; 皮燕; 龙春燕; 孙梦娇; 陈雪; 李敬诚; 张莉莉

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6-姜烯酚通过下调TLR4/NF-κB通路抑制血管平滑肌细胞增殖

基础医学 | 更新时间：2026-01-09

- 6-姜烯酚通过下调TLR4/NF-κB通路抑制血管平滑肌细胞增殖
- 6-shogaol suppresses vascular smooth muscle cells proliferation by inhibiting TLR4/NF-κB signaling pathway
- 陆军军医大学学报 2016年38卷第3期页码：245-250
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  中图分类号： R282.71;R322.12;R329.28
- 纸质出版：2016
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陈磊,胡子成,周毅,张明杰,王旭,皮燕,龙春燕,孙梦娇,陈雪,李敬诚,张莉莉.6-姜烯酚通过下调TLR4/NF-κB通路抑制血管平滑肌细胞增殖[J].陆军军医大学学报(原第三军医大学学报),2016,38(03):245-250.

　Chen Lei,Hu Zicheng,Zhou Yi,Zhang Mingjie,Wang Xu,Pi Yan,Long Chunyan,Sun Mengjiao,Chen Xue,Li Jingcheng,Zhang Lili.6-shogaol suppresses vascular smooth muscle cells proliferation by inhibiting TLR4/NF-κB signaling pathway[J].J Amry Med Univ (J Third Mil Med Univ),2016,38(03):245-250.
陈磊,胡子成,周毅,张明杰,王旭,皮燕,龙春燕,孙梦娇,陈雪,李敬诚,张莉莉.6-姜烯酚通过下调TLR4/NF-κB通路抑制血管平滑肌细胞增殖[J].陆军军医大学学报(原第三军医大学学报),2016,38(03):245-250. DOI：

　Chen Lei,Hu Zicheng,Zhou Yi,Zhang Mingjie,Wang Xu,Pi Yan,Long Chunyan,Sun Mengjiao,Chen Xue,Li Jingcheng,Zhang Lili.6-shogaol suppresses vascular smooth muscle cells proliferation by inhibiting TLR4/NF-κB signaling pathway[J].J Amry Med Univ (J Third Mil Med Univ),2016,38(03):245-250. DOI：

摘要

目的探讨6-姜烯酚对血管平滑肌细胞（vascular smooth muscle cells，VSMCs）增殖的影响及其可能的分子机制。方法组织贴块法培养野生型（wild type，WT）和Toll样受体4敲除（Toll-like receptor 4 knockout， TLR4

-/-

）小鼠原代VSMCs。应用血小板源性生长因子(platelet derived growth factor

PDGF)诱导VSMCs增殖。分别应用TLR4配体脂多糖（LPS）和TLR4抑制剂（Eritoran，E5564）激活和抑制TLR4，观察TLR4在VSMCs增殖中的作用。加入6-姜烯酚刺激，观察对VSMC增殖以及对TLR4表达的影响。结果 6-姜烯酚呈浓度依赖性地抑制PDGF诱导的VSMCs增殖(P0.05)；PDGF和LPS均可激活TLR4并促进野生VSMCs的增殖（P0.05），TLR4基因敲除抑制PDGF和LPS诱导的VSMCs增殖（P0.05）；6-姜烯酚下调VSMCs中TLR4表达（P0.05）。6-姜烯酚下调VSMCs中TLR4下游蛋白NF-κB的表达（P0.05）。结论 6-姜烯酚通过下调TLR4/NF-κB通路抑制血管平滑肌细胞增殖。

Abstract

Objective To determine the effect of 6-shogaol on the proliferation in vascular smooth muscle cells (VSMCs) and investigate its underlying mechanism. Methods VSMCs were isolated from the thoracic aorta of wild-type (WT

C57BL/6J background) and Toll-like receptor 4 knockout (TLR4

-/-

C57BL/6J background) mice. Platelet derived growth factor (PDGF) was used to induce the proliferation in the obtained VSMCs. In order to observe the effect of TLR4 in VSMCs

LPS was a

dded to the medium of TLR4

-/-

and WT VSMCs respectively to activate TLR4

while TLR4 inhibitor

E5564 was used to inhibit the expression of TLR4. 6-shogaol was added to observe its effect on the proliferation and TLR4 expression in VSMCs. Results 6-shogaol inhibited the proliferation of VSMCs in a dose-dependent manner (P＜0.05). Both PDGF and LPS activated TLR4 and promoted the cell proliferation (P＜0.05)

but knockout of TLR4 gene suppressed the induced proliferation (P＜0.05). 6-shogaol also inhabited the expression of TLR4 and its downstream protein

NF-κB (P＜0.05). Conclusion 6-shogaol suppresses the proliferation in VSMCs via inhibiting TLR4/NF-κB signaling pathway.

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