Objective      To determine the effect of Gsdermin A3 (Gsdma3) on the expression of Krt6b.       Methods      Q-PCR

RT-PCR

Western blotting and immunohistochemical staining were used to detect the expression of Krt6b at mRNA and protein levels in Gsdma3 mutant mice (AE) and C57BL/6J wild-type mice (WT) at 3 time points in mouse hair follicle cycle (anagen: 8 d postnatally

catagen: 16 d postnatally

telogen: 25 d postnatally). Immunofluorescence assay was also used to determine the effect of the adenoviruses carrying Gsdma3 gene (AdGsdma3) or control gene (AdRFP) on the expression of Krt6b in AE and WT mice.       Results      The expression level of Krt6b was obviously higher in Gsdma3 mutant mice than WT mice (P0.05). At P16

the expression of Krt6b reached the highest level

while on P25 it reduced to the lowest level. Immunofluorescence assay

real-time PCR and Western blotting showed that the expression of Krt6b was decreased in Gsdma3 mutant mice after Gsdma3 expression rescue. Meanwhile

immunofluorescence assay showed that the expression of Krt6b was also decreased in WT mice with Gsdma3 over-expression.       Conclusion      Gsdma3 inhibits the expression of Krt6b in mouse keratinocytes.