Objective      To determine the effect of long non-coding RNA H19 (lncRNA H19) on the expression of miR-124-3p and the proliferation of hepatocellular carcinoma cells.       Methods      Real-time PCR was used to detect the expression of lncRNA H19 and miR-124-3p in hepatocellular carcinoma cell lines (HepG2 and SMMC7721) and clinical specimens. After over-expressing lncRNA H19 in HepG2 and SMMC7721 cells

real-time PCR was carried out to detect the expression of miR-124-3p and its target gene， cyclinD1

and Western blotting was used to detect the expression of cyclinD1 at protein level. Cell proliferation was assessed by CCK-8 assay. After over-expressing lncRNA H19 and transferring antagomiR-124-3p into SMMC7721 and HepG2 cells in the meantime

the expression of cyclinD1 and the proliferation of cells were detected again.       Results      lncRNA H19 and miR-124-3p had low expression in tumor tissues and hepatocellular carcinoma cell lines (P0.05). Compared with the control group

the expression of miR-124-3p was increased in SMMC7721 and HepG2 cells after over-expressing lncRNA H19 (P0.05). The protein and mRNA levels of cyclinD1 were decreased in the over-expressing cells (P0.05). Over-expression of lncRNA H19 suppressed the proliferation of HepG2 and SMMC7721 cells (P0.05). However

overexpressing lncRNA H19 in the cells with antagomiR-124-3p transferring relieved the suppression of proliferation (P0.05).       Conclusion      lncRNA H19 suppresses cyclinD1 expression and proliferation  hepatocellular carcinoma cells through enhancing miR-124-3p.