Objective      To investigate the effect of RNAi-mediated glucose regulated protein 94 (Grp94) gene silencing on migration and invasion of human esophageal cancer ECA109 cells and the possible mechanism．       Methods      Chemically synthesized siRNA targeting Grp94 gene was transferred into ECA109 cells by Liopfectamine RNAIMAX

and ECA109 cells transfected with negative control siRNA were used as controls. The mRNA and protein expressions of Grp94

matrix metalloproteinase 2 (MMP2) and matrix metalloproteinase 9 (MMP9) were evaluated by real-time PCR and Western blot

respectively. The effect of Grp94 silencing on the migration and invasion of ECA109 cells was evaluate by Transwell migration assay and Matrigel invasion assay．       Results      The expressions of Grp94 mRNA and protein were significantly down-regulated in ECA109 cells transfected with Grp94-targeting siRNA as compared with the control group (P0.01)

as well as the expressions of MMP2 and MMP9 mRNA and protein (P0.05). The migration and invasion of ECA109 cells transfected with Grp94-targeting siRNA were lower than those of ECA109 cells transfected with negative control siRNA (P0.05)．      Conclusion      Grp94 gene silencing can significantly inhibit the migration and invasion of ECA109 cells through influencing the expressions of MMP2 and MMP9.