Objective      To explore the expression of Golgi matrix protein 130 (GM130) in different differentiated human gastric carcinoma cell lines and investigate the effect of small interference RNA-mediated GM130 gene silencing on the cell proliferation

invasion and migration in vitro.       Methods      Three different differentiated gastric carcinoma cell lines (MKN-28

SGC-7901 and MKN-45) were cultured in vitro. Western blot analysis and RT-PCR were carried out to screen MKN-45 cells and SGC-7901cells with high GM130 expression. GM130-targeting siRNA was designed

chemically synthesized and transferred into MKN-45 cells. The effects of GM130 gene silencing on the proliferation

migration and invasion of MKN-45 cells were analyzed by MTT assay

Transwell migration assay and Matrigel invasion assay

respectively. The expression of matrix metalloproteinase 2 (MMP2) and matrix metalloproteinase 9 (MMP9) were also detected by Western blotting.       Results      MKN-45 cells and SGC-7901 cells had high expression of GM130 at mRNA and protein levels. Western blotting and qRT-PCR showed that GM130-siRNA-519 significantly decreased the GM130 expression in the low differentiated MKN-45cells (P0.05). The cell proliferation

migration and invasion of MKN-45 cells transfected with GM130-siRNA were suppressed (P0.05)

and the protein levels of MMP-2 and MMP-9 were significantly decreased (P0.05).        Conclusion      Small interference RNA-mediated GM130 gene silencing can significantly inhibit the proliferation

migration and invasion in human gastric cancer cell line MKN-45.