Objective      To observe the migration ability of bone marrow mesenchymal stem cells (BMSCs) after the treatment of the serum derived from severely burned rats

and determine the role of PI3K/Akt signaling pathway in the process.       Methods      After severely burned rat model was established

the rat serum was derived. (1) After the BMSCs were treated by rat serum at volume fraction of 10% for different time periods

Transwell chambers test was used to detect the migration of BMSCs

and Western blotting to measure the expression of intracellular phosphorylation p38 (p-p38) and phosphorylated Akt (p-Akt). (2) BMSCs cells were randomly divided into 4 groups of cells

that is

control group (treated by 10% fetal bovine serum)

serum group (containing 10% serum from burned rats in general rat serum)

serum +inhibitor group (containing 10% serum from burned rats +10 μmol/L LY294002)

serum +agonist group (containing 10% serum from burned rats +200 ng/mL IGF-1). Transwell chamber test was used to detect cell migration.       Results      (1) In 0

1

3

6

12 and 24 h after burned serum treatment

the quantity of BMSCs cells migration was 0

1.0±0.7

14.6±4.6

45.0±6.4

82.8±8.3 and 122.2±11.4 respectively. Cell migration was observed since 3 h

the number of migrated cells in 6

12 and 24 h was significantly larger than that of 3 h (P0.01). After burned serum treatment for 0

1

3

6

12 and 24 h

the expression level of p-p38 was 1.02±0.50

1.11±0.17

1.04±0.23

1.02±0.2

1.16±0.22 and 1.00±0.19 respectively

and no significant difference was seen in the expression levels among these time points (P0.05). The expression level of p-Akt was 0.59±0.08

0.65±0.06

0.83±0.22

1.10±0.21

1.22±0.19 and 1.62±0.30 respectively for 6

12 and 24 h treatment

and the levels were significantly higher than that of 0 h (instantly after serum adding

P0.05)

and that of 12 and 24 h was significantly higher than that of 3 h (P0.05). (2) In the control group

burned serum group

the burned serum+ inhibitors

burned serum+ agonist group after 24 hours’ treatment

the number of BMSCs migration was larger in the burned serum group than the control group (P0.01)

in the burned serum +agonist group than the burned serum group (P0.01)

and was the lowest in the burned serum+ inhibitor group (P0.01).       Conclusion      The serum derived from severe burned rats promotes the migration of BMSCs by PI3K/Akt signal pathway.