Objective        To systematically investigate the dysregulation of long non-coding RNAs (lncRNAs) and mRNAs profiles and mechanism for apoptosis induction after co-culture of SW480 cells with Clostridium butyricum (CB).       Methods       After SW480 cells were co-cultured with 1.5×10

7

CFU/mL CB for 48 h

the total RNA of the cells were extracted and analyzed by Agilent lncRNAs microarray. The SW480 cells without CB treatment served as control. The target genes of dysregulated lncRNAs and dysr

egulated mRNAs were analyzed by DAVID 6.7 software. Hoechst 33258 (bisbenzimlde) and Annexin V-FITC/PI were used to detect the apoptosis in SW480 cells.       Results       Compared with the control cells

there were 50 up-regulated lncRNAs and 152 down-regulated lncRNAs

while 738 up-regulated mRNAs and 1 088 down-regulated mRNAs found in the CB-treated cells. After pathway enrichment analysis

these lncRNAs and mRNAs were related to tumor associated signaling pathways

including MAPK signaling

cytokine-cytokine

Jak-STAT signaling

NF-κB activation

VEGF signaling and p53 signaling.       Conclusion       CB promotes the apoptosis in SW480 cells by dysregulating long non-coding RNAs.