一种昆明种属来源的视网膜变性小鼠同源导入C57BL/6J小鼠近交系的表型研究

姚璐; 齐林嵩; 安晶; 张作明; 张磊

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一种昆明种属来源的视网膜变性小鼠同源导入C57BL/6J小鼠近交系的表型研究

论著 | 更新时间：2026-01-09

- 一种昆明种属来源的视网膜变性小鼠同源导入C57BL/6J小鼠近交系的表型研究
- Phenotype of congenic inbred strain of C57BL/6J mice and retinal degeneration mice stemmed from Kunming mice
- 陆军军医大学学报 2015年37卷第12期页码：1183-1187
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  中图分类号： R-334;R394.1;R774.13
- 纸质出版：2015
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姚璐,齐林嵩,安晶,张作明,张磊.一种昆明种属来源的视网膜变性小鼠同源导入C57BL/6J小鼠近交系的表型研究[J].陆军军医大学学报(原第三军医大学学报),2015,37(12):1183-1187.

　Yao Lu,Qi Linsong,An Jing,Zhang Zuoming,Zhang Lei.Phenotype of congenic inbred strain of C57BL/6J mice and retinal degeneration mice stemmed from Kunming mice[J].J Amry Med Univ (J Third Mil Med Univ),2015,37(12):1183-1187.
姚璐,齐林嵩,安晶,张作明,张磊.一种昆明种属来源的视网膜变性小鼠同源导入C57BL/6J小鼠近交系的表型研究[J].陆军军医大学学报(原第三军医大学学报),2015,37(12):1183-1187. DOI：

　Yao Lu,Qi Linsong,An Jing,Zhang Zuoming,Zhang Lei.Phenotype of congenic inbred strain of C57BL/6J mice and retinal degeneration mice stemmed from Kunming mice[J].J Amry Med Univ (J Third Mil Med Univ),2015,37(12):1183-1187. DOI：

摘要

目的将本实验室发现的一种昆明种属来源的自发性遗传性视网膜变性小鼠(暂命名为KM

rdf/rdf

小鼠)的致病基因同源导入C57BL/6J小鼠，培育一种新的近交系小鼠（暂命名为B6

rdf/rdf

小鼠），并对B6

rdf/rdf

小鼠进行眼部表型的发育学研究。方法采用眼底照相、眼底荧光素血管造影、组织形态学和视网膜电图观测不同年龄B6

rdf/rdf

小鼠的眼部表型。结果眼底照相和荧光素血管造影发现B6

rdf/rdf

小鼠表现为进行性视网膜血管退化及脱色素改变，视网膜组织形态学表现为外核层在P14显著变薄，外核层和外网状层至P21已基本消失，视网膜电图自P14已记录不到明显波形。结论 B6

rdf/rdf

f小鼠眼部表型呈快速进行性改变，与KM

rdf/rdf

小鼠表型相似。

Abstract

Objective To cross spontaneously hereditary retinal degeneration mice (temporarily designated as KM

rdf/rdf

mice) derived from Kunming mice with C57BL/6J mice to establish a congenic inbred strain (temporarily designated as the mice)

and study the ocular phenotype of the mice. Methods Fundus photography

fundus fluorescein angiography

tissue morphology and electroretinography (ERG) were performed to observe the ocular phenotype of B6

rdf/rdf

mice at different ages. Results The fundus photography and fundus fluorescein angiography showed progressive retinal vascular de

generation and depigmentation. The tissue morphology showed that the outer nuclear layer was significantly thinned at P14

and the outer nuclear layer and outer plexiform layer almost disappeared at 21 d post born. No obvious waveform was observed from 14 d after born in the ERG. Conclusion The ocular phenotype of B6

rdf/rdf

mice was similar to that of KM

rdf/rdf

mice

which indicates that B6

rdf/rdf

mice can be used as an retinitis pigmentosa mouse model.

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