宫颈鳞癌组织中HPV E6与野生型p53和PinX1表达的关系及其临床意义

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宫颈鳞癌组织中HPV E6与野生型p53和PinX1表达的关系及其临床意义

论著 | 更新时间：2026-01-09

- 宫颈鳞癌组织中HPV E6与野生型p53和PinX1表达的关系及其临床意义
- Correlation of HPV E6 with wild type p53 and PinX1 in cervical squamous carcinoma and clinical significance
- 陆军军医大学学报 2015年37卷第8期页码：782-786
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  中图分类号： R362;R730.43;R737.33
- 纸质出版：2015
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王婉,黄团明,王滨,闫晓欢,郑英如.宫颈鳞癌组织中HPV E6与野生型p53和PinX1表达的关系及其临床意义[J].陆军军医大学学报(原第三军医大学学报),2015,37(08 ):782-786.　Wang Wan,Huang Tuanming,Wang Bin,Yan Xiaohuan,Zheng Yingru.Correlation of HPV E6 with wild type p53 and PinX1 in cervical squamous carcinoma and clinical significance[J].J Amry Med Univ (J Third Mil Med Univ),2015,37(08 ):782-786.

王婉, 黄团明, 王滨, et al. Correlation of HPV E6 with wild type p53 and PinX1 in cervical squamous carcinoma and clinical significance[J]. Journal of Army Medical University, 2015, 37(8): 782-786.
王婉,黄团明,王滨,闫晓欢,郑英如.宫颈鳞癌组织中HPV E6与野生型p53和PinX1表达的关系及其临床意义[J].陆军军医大学学报(原第三军医大学学报),2015,37(08 ):782-786.　Wang Wan,Huang Tuanming,Wang Bin,Yan Xiaohuan,Zheng Yingru.Correlation of HPV E6 with wild type p53 and PinX1 in cervical squamous carcinoma and clinical significance[J].J Amry Med Univ (J Third Mil Med Univ),2015,37(08 ):782-786. DOI：

王婉, 黄团明, 王滨, et al. Correlation of HPV E6 with wild type p53 and PinX1 in cervical squamous carcinoma and clinical significance[J]. Journal of Army Medical University, 2015, 37(8): 782-786. DOI：

摘要

目的探讨宫颈鳞癌、宫颈鳞癌癌旁及正常宫颈组织中HPV E6、野生型p53及PinX1表达及其关系。方法收集第三军医大学大坪医院野战外科研究所妇产科2013年3月至2014年2月17例宫颈鳞癌患者宫颈鳞癌、宫颈鳞癌癌旁组织，选取10例正常宫颈组织作为对照。采用Real-Time PCR、Western blot、免疫组织化学方法检测3组组织中HPV E6、野生型p53和PinX1的mRNA、蛋白的表达及分布。结果宫颈鳞癌组织中mRNA及蛋白水平结果均显示HPV E6的表达水平显著高于癌旁组织（P0.01），而野生型p53和PinX1的表达水平明显低于癌旁及正常组织（P0.05）。相关性分析提示：宫颈鳞癌组织中野生型p53(R=-0.605，P0.01)和PinX1(R=-0.496，P0.05)表达水平的下降与HPV E6的表达水平升高存在负相关，野生型p53与PinX1的表达呈正相关(R=0.824，P0.01)。结论宫颈鳞癌组织中HPV E6表达上调与野生型p53、PinX1的表达负相关，PinX1的表达与野生型p53密切相关，HPV E6可能通过抑制野生型p53、PinX1导致宫颈鳞癌的发生、发展。

Abstract

Objective To investigate the expression and relationship of human papillomavirus (HPV) E6

wild type p53

and PinX1 in cervical squamous carcinoma tissues

adjacent tissues

and normal cervical tissues. Methods Seventeen cases of cervical squamous carcinoma and adjacent tissues were collected from Department of Obstetrics and Gynecology

Daping Hospital of the Third Military Medical University during March 2013 to February 2014

and 10 cases of normal cervical tissues were selected as control. Real-time PCR

Western blotting and immunohistochemistry assay were used to detect the expression and distribution of HPV E6

wild type p53

and PinX1 in cervical squamous carcinoma tissues

adjacent tissues

and normal cervical tissues. Results The mRNA and protein levels of HPV E6 in the cervical squamous carcinoma tissues were significantly higher than those in the adjacent mucosa (P0.05)

and the expression levels of wild type p53 and PinX1 were dramatically decreased compared with adjacent and normal mucosa (P0.05). HPV E6 had a negative correlation with the decreased expression levels of wild type p53 (R=-0.605

P0.01) and PinX1 (R=-0.496

P0.05) in cervical squamous carcinoma

however

a positive correlation was seen between the expression levels of wide type p53 and PinX1 (R=0.824

P0.01). Conclusion HPV E6 expression is negatively related with wild type p53 and PinX1 in cervical squamous carcinoma

and PinX1 is closely related with the expression of wild type p53. HPV E6 may result in the carcinogenesis and development of cervical squamous carcinoma through inhibiting wild type p53 and PinX1.

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