Objective      To investigate the effect of sperm-associated antigen 9 (SPAG9) over-expression on the proliferation

cell cycle and migration in human prostate cancer cell line PC3 and the relevant molecular mechanism.       Methods      PC3 prostate cancer cells were transformed with plasmid M29-SPAG9 and plasmid M29

and qRT-PCR and Western blotting were used to detect the SPAG9 expression levels. The cell proliferation was detected by CCK-8 assay. The ce

ll cycle distribution was analyzed by flow cytometry. Transwell migration assay was used to analyze cell migration ability.       Results      The results of qRT-PCR and Western blot analysis indicated that the SPAG9 expression in the M29-SPAG9 over-expression group was higher than that of the M29 control group (20.776 3±3.080 9 vs 1.010 2±0.178 4

P0.01). Compared with the M29 control group

the cell proliferation activity was significantly higher in the SPAG9 over-expression group (P0.05)

and the G

2

/S phase proportion was increased (P0.05). Transwell migration assay indicated that the cell migration ability of the M29-SPAG9 over-expression group was significantly higher than that of the M29 control group (88.078±4.509 vs 54.333±4.582

P0.05).       Conclusion      Over-expression of SPAG9 promotes the proliferation

cell cycle and migration in human prostate cancer cell line PC3 through ERK and JNK signaling pathway.