Objective        To investigate the expression of Munc18-1 protein in the inner hair cells of mouse cochlea and identify its location.      Methods        Thirty C57 mice (60 cochleae) were randomly detected with Munc18-1 antibody or CtBP2+Munc18-1 double antibodies by immunofluorescence assay and confocal laser imaging technique to detect the expression and location of Munc18-1 protein in the cochlea inner hair cells.      Results      Laser scanning confocal imaging indicated that the Munc18-1 protein was highly expressed in the cytoplasm of the inner hair cells

and Munc18-1 protein was aggregated close to the synaptic protein CtBP2.      Conclusion        Munc18-1 protein exists in the mouse cochlea inner hair cells

and aggregates with synaptic protein CtBP2. It might be closely involved in the maintenance of synaptic vesicles fusion and exocytosis.