Objective      To manufacture surface-modified silicone rubber (SR) materials with microgroove surface

detect the surface physicochemical properties of the SR materials before and after modification

and evaluate the cytocompatibility.       Methods      Using a microgroove graphic   template

surface-modified SR materials with microgroove surface were manufactured successfully

and carbon ion implantation with uniform dose was performed. Scanning electron microscope (SEM)

atomic force microscope (AFM)

and water contact angle test were used to evaluate the surface physicochemical properties of all the materials. The influence of surface microtopography on human dermal fibroblasts was observed by cell adhesion experiment

CCK-8 assay and Western blot assay.       Results      AFM images showed that obvious microtopography difference existed among different materials. Parallel microgrooves were found on the surfaces of patterned SR (P-SR) and patterned carbon ion implanted SR (PC-SR)

while carbon ion implanted SR (C-SR) surface showed irregular larger peaks and deeper valleys. The water contact angle results showed that parallel microgrooves could increase the water contact angle of the materials. Cell adhesion and proliferation experiments indicated that cell adhesion and growth were slightly decreased after surface microgroove modification. The immunofluorescence results indicated that human dermal fibroblasts were aligned on the P-SR and PC-SR. Western blot results showed that the expressions of cell adhesion associated proteins Talin and Zyxin were higher on the C-SR and PC-SR than on the SR (P0.05).       Conclusion      Microgroove surface of SR materials can influence the surface physicochemical properties and the cell proliferation and arrangement

and has positive effect on reducing capsular contracture.