<sup>131</sup>I-CD133mAb诱导CD133<sup>+</sup>HepG2肝癌干细胞间质-上皮逆转分化

史燕龙; 李亚军; 康强强; 侯妍利; 刘锐; 李少林

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¹³¹I-CD133mAb诱导CD133⁺HepG2肝癌干细胞间质-上皮逆转分化

论著 | 更新时间：2026-01-09

- ¹³¹I-CD133mAb诱导CD133⁺HepG2肝癌干细胞间质-上皮逆转分化
- ¹³¹I-CD133mAb induces mesenchymal-epithelial transition in CD133⁺ HepG2 liver cancer stem cells
- 陆军军医大学学报 2015年37卷第11期页码：1086-1090
- 作者机构：
- 作者简介：
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- DOI：
  中图分类号： R730.23;R735.7;R817.1
- 纸质出版：2015
- 稿件说明：
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史燕龙,李亚军,康强强,侯妍利,刘锐,李少林.131I-CD133mAb诱导CD133+HepG2肝癌干细胞间质-上皮逆转分化[J].陆军军医大学学报(原第三军医大学学报),2015,37(11):1086-1090.

　Shi Yanlong,Li Yajun,Kang Qiangqiang,Hou Yanli,Liu Rui,Li Shaolin.131I-CD133mAb induces mesenchymal-epithelial transition in CD133+ HepG2 liver cancer stem cells[J].J Amry Med Univ (J Third Mil Med Univ),2015,37(11):1086-1090.
史燕龙,李亚军,康强强,侯妍利,刘锐,李少林.131I-CD133mAb诱导CD133+HepG2肝癌干细胞间质-上皮逆转分化[J].陆军军医大学学报(原第三军医大学学报),2015,37(11):1086-1090. DOI：

　Shi Yanlong,Li Yajun,Kang Qiangqiang,Hou Yanli,Liu Rui,Li Shaolin.131I-CD133mAb induces mesenchymal-epithelial transition in CD133+ HepG2 liver cancer stem cells[J].J Amry Med Univ (J Third Mil Med Univ),2015,37(11):1086-1090. DOI：

摘要

目的研究

131

I-CD133mAb可诱导CD133

HepG2肝癌干细胞间质-上皮逆转分化（mesenchymal-epithelial transition，MET）及可能分子机制。方法 ①用免疫磁珠分选法分选HepG2人肝癌细胞

采用流式细胞术检测分选前后CD133的表达率，体外成球和体内成瘤实验鉴定其干细胞特性。②氯胺T法制备

131

I标记CD133mAb并用γ免疫计数器检测标记率、放化纯度。用不同剂量（0、2.4、4.8、9.6 MBq）的

131

I-CD133mAb作用CD133

HepG2细胞，用Transwell侵袭实验检测侵袭能力，Western blot检测E-cadherin、N-cadherin和Vimentin 的蛋白表达水平。结果流式细胞术显示分选前后CD133表达率分别为（7.21±0.05）%和（95.26±0.05）%。CD133

细胞体外成球和体内成瘤能力强于CD133

细胞。γ免疫计数器检测

131

I-CD133抗体标记率为86.97%，放化纯度为98.84%。Transwell侵袭实验示

131

I-CD133mAb各处理组（2.4、4.8、9.6 MBq）24 h细胞穿过Matrigel胶的细胞分别为（107.7±3.2）、（76.3±2.5）、（44.0±3.0）/视野，显著少于对照组（0 MBq，P0.01）。Western blot结果显示，与对照组（0 MBq）相比，

131

I-CD133mAb（2.4、4.8、9.6 MBq）处理组N-cadherin和Vimentin的蛋白表达量降低（P0.01），E-cadherin的蛋白表达量增加（P0.01）。结论在体外

131

I-CD133mAb作用于人肝癌CD133

HepG2细胞可诱导间质-上皮逆转分化。

Abstract

Objective To study the role of

131

I-labeled CD133 monoclonal antibody (

131

I-CD133mA

) in inducing mesenchymal-epithelial transition (MET) of CD133

HepG2 liver cancer stem cells and possible molecular mechanism. Methods Magnetic-activated cell sorting (MACS) was used to isolate CD133

HepG2 cells and CD133

HepG2 cells. Flow cytometry (FCM) was used to detect the expression of CD133 before and after cell sorting. The stem cell characteristics of the sorted CD133

cells were validated by sphere-forming assay and in vivo transplantation tumor experiment in BALB/c mice.

131

I-CD133mA was prepared with a chloramine-T method. After that

the stability was evaluated

and an γ immune counter was used to detect the labeling rate and the radiochemical purity. Different doses (0

2.4

4.8 and 9.6 MBq) of

131

I-CD133mAb were applied to the CD133

HepG2 cells. Transwell chamber test was adopted to detect cell invasion

and Western blotting was employed to detect the expressions of E-cadherin

N-cadherin and Vimentin. Results The sorted CD133

HepG2 cells showed higher purity (95.26±0.05)% as compared with (7.21±0.05)% before cell sorting

and had higher sphere forming ability and tumor forming ability as compared with CD133

HepG2 cells. The labeling ratio of

131

I-CD133mA was 86.97%

and the radiochemical purity was 98.84%. Transwell chamber test results showed that in the

131

I-CD133mAb treatment groups (2.4

4.8 and 9.6 MBq)

Matrigel-permeating cells were (107.7±3.2)/high-power field

(76.3±2.5)/high-power field

and (44.0±3.0)/high-power field

respectively

which were significantly fewer than (130.0±3.6)/high-power field in the control group (0 MBq) (P0.01). The results of Western blot indicated that compared with the control group

the expression of N-cadherin and Vimentin in the

131

I-CD133mAb treatment groups was decreased significantly (P0.01)

while the expression of E-cadherin increased significantly (P0.01). Conclusion In vitro applicat

ion of

131

I-CD133mAb can effectively induce the MET phenotypic change in CD133

HepG2 liver cancer stem cells.

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131I-CD133mAb诱导CD133+HepG2肝癌干细胞间质-上皮逆转分化

131I-CD133mAb induces mesenchymal-epithelial transition in CD133+ HepG2 liver cancer stem cells

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摘要

Abstract

关键词

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references

¹³¹I-CD133mAb诱导CD133⁺HepG2肝癌干细胞间质-上皮逆转分化

¹³¹I-CD133mAb induces mesenchymal-epithelial transition in CD133⁺ HepG2 liver cancer stem cells