Objective      To investigate the effect of Lysine-Cyclic RGD (LcRGD) peptide

which is new surface modifier based on biological ligand and physical positive charge

on the adhesion

proliferation and differentiation of mesenchymal stem cells (MSCs).       Methods      The hydrophilicity of LcRGD peptide-modified cover glass was measured via contact angle that was detected with an optical tensiometer.  The adhesion ability of MSCs to LcRGD peptide was measured by centrifugal cell adhesion assay and vortex cell adhesion assay. The proliferation and differentiation ability of the MSCs was detected by CCK-8 assay and Western blotting.       Results      LcRGD peptide-modified cover glass possessed contact angle (29.33±4.06)° lower than (63.72±8.65)° of cRGD peptide-modified cover glass

indicating better hydrophilicity (P0.01). The results of centrifugal cell adhesion assay and vortex cell adhesion assay confirmed the LcRGD peptide had better cell adhesion (P0.01) than cRGD peptide from the initial stage of cell adhesion. The results of CCK-8 assay and Western blotting showed that the MSCs seeded on LcRGD peptide-modified decalcified bone matrix (DBM) showed higher proliferation activity as compared with cRGD peptide-modified DBM (P0.05)

and Runx2 and OCN protein levels were significantly increased.       Conclusion      LcRGD peptide holds satisfactory cell adhesion

biocompatibility and osteoinduction

suggesting it is a preferable modifier for tissue engineered bone scaffold.